Identification of an effective early signaling signature during neo-vasculogenesis in vivo by ex vivo proteomic profiling

PloS One
Rokhsareh RohbanDirk Strunk


Therapeutic neo-vasculogenesis in vivo can be achieved by the co-transplantation of human endothelial colony-forming progenitor cells (ECFCs) with mesenchymal stem/progenitor cells (MSPCs). The underlying mechanism is not completely understood thus hampering the development of novel stem cell therapies. We hypothesized that proteomic profiling could be used to retrieve the in vivo signaling signature during the initial phase of human neo-vasculogenesis. ECFCs and MSPCs were therefore either transplanted alone or co-transplanted subcutaneously into immune deficient mice. Early cell signaling, occurring within the first 24 hours in vivo, was analyzed using antibody microarray proteomic profiling. Vessel formation and persistence were verified in parallel transplants for up to 24 weeks. Proteomic analysis revealed significant alteration of regulatory components including caspases, calcium/calmodulin-dependent protein kinase, DNA protein kinase, human ErbB2 receptor-tyrosine kinase as well as mitogen-activated protein kinases. Caspase-4 was selected from array results as one therapeutic candidate for targeting vascular network formation in vitro as well as modulating therapeutic vasculogenesis in vivo. As a proof-of-principle, casp...Continue Reading


Jan 30, 2015·American Journal of Veterinary Research·Margaret M SalterAnne A Wooldridge
Nov 12, 2015·Journal of Translational Medicine·Sandra Laner-PlambergerEva Rohde
Mar 14, 2017·Stem Cells International·Rokhsareh Rohban, Thomas Rudolf Pieber
May 2, 2017·The Journal of Clinical Investigation·Robert P Hebbel


May 8, 1980·Nature·A R Means, J R Dedman
Jan 1, 1995·Annual Review of Cell and Developmental Biology·W Risau, I Flamme
Dec 24, 1996·Biochemical and Biophysical Research Communications·D Mukhopadhyay, H I Akbarali
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J S SchechnerJ S Pober
Oct 26, 2000·Nature·P MeierG Evan
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Inmaculada SeguraHans H Riese
Jul 9, 2002·Apoptosis : an International Journal on Programmed Cell Death·M Chen, J Wang
Apr 23, 2003·The Journal of Molecular Diagnostics : JMD·Chris CheadleKevin G Becker
Jun 20, 2003·Apoptosis : an International Journal on Programmed Cell Death·T M CasertaT L Brown
May 5, 2004·The Journal of Cell Biology·Junichi HitomiMasaya Tohyama
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amal NadiriMaya Saleh
Nov 23, 2007·Biochemical Society Transactions·K KersseP Vandenabeele
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·John G WalshSeamus J Martin
Sep 5, 2008·Current Protocols in Stem Cell Biology·Laura E MeadDavid A Ingram
Jan 6, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Dörte ScharnerStefanie Dimmeler
May 7, 2009·Molecular Cancer Therapeutics·Luigi MorettiBo Lu
Aug 1, 2009·Investigative Ophthalmology & Visual Science·Zong-Mei BianVictor M Elner
May 20, 2011·Nature·Peter Carmeliet, Rakesh K Jain
Jun 15, 2011·Cell Transplantation·Krisztina SzökeJan E Brinchmann
Jul 14, 2011·Journal of Cellular Biochemistry·Kateřina KuželováBarbora Brodská
Jan 17, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gabriel SollbergerHans-Dietmar Beer
May 23, 2012·Methods in Molecular Biology·Nicole A HofmannDirk Strunk
Aug 3, 2012·Cold Spring Harbor Perspectives in Biology·Hyung Don Ryoo, Andreas Bergmann

Related Concepts

HSPB1 gene
PRKDC gene
ErbB-2 Receptor
Flow Cytometry
Biochemical Pathway
Calcium [EPC]
VIM gene

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Blood Brain Barrier Regulation in Health & Disease

The BBB is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the BBB and changes in permeability allows pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research on BBB regulation in health and disease.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis