Identification of an endogenous retroviral envelope gene with fusogenic activity and placenta-specific expression in the rabbit: a new "syncytin" in a third order of mammals.

Retrovirology
O HeidmannT Heidmann

Abstract

Syncytins are envelope genes of retroviral origin that have been co-opted by the host to mediate a specialized function in placentation. Two of these genes have already been identified in primates, as well as two distinct, non orthologous genes in rodents. Here we identified within the rabbit Oryctolagus cuniculus-which belongs to the lagomorpha order- an envelope (env) gene of retroviral origin with the characteristic features of a bona fide syncytin, that we named syncytin-Ory1. An in silico search for full-length env genes with an uninterrupted open reading frame within the rabbit genome first identified two candidate genes that were tested for their specific expression in the placenta by quantitative RT-PCR of RNA isolated from a large set of tissues. This resulted in the identification of an env gene with placenta-specific expression and belonging to a family of endogenous retroelements present at a limited copy number in the rabbit genome. Functional characterization of the identified placenta-expressed env gene after cloning in a CMV-driven expression vector and transient transfection experiments, demonstrated both fusogenic activity in an ex vivo cell-cell fusion assay and infectivity of pseudotypes. The receptor for th...Continue Reading

References

Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J E RaskoA D Miller
Feb 22, 2003·Biochimie·Didier Nègre, François-Loïc Cosset
Nov 1, 1961·The American Journal of Anatomy·J F LARSEN
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sandra BlaiseThierry Heidmann
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·François MalletBernard Mandrand
Jan 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anne DupressoirThierry Heidmann
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Marianne MangeneyThierry Heidmann
Nov 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Cécile EsnaultThierry Heidmann
Jul 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anne DupressoirThierry Heidmann

❮ Previous
Next ❯

Citations

Mar 5, 2013·Trends in Genetics : TIG·Pablo S AguilarMelissa Wong
Oct 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anne DupressoirThierry Heidmann
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Guillaume CornelisThierry Heidmann
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Harmit Singh Malik
Jul 4, 2012·Briefings in Functional Genomics·Deena Emera, Günter P Wagner
Aug 14, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gkikas MagiorkinisAris Katzourakis
Aug 14, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christian LavialleThierry Heidmann
Jul 24, 2010·Genome Research·Henrik Kaessmann
Jan 27, 2012·Journal of Virology·Abdou AkkoucheMarie Fablet
Aug 29, 2012·International Journal of Evolutionary Biology·Domitille ChalopinJean-Nicolas Volff
Jun 2, 2011·Mobile DNA·Keith R Oliver, Wayne K Greene
Oct 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Guillaume CornelisThierry Heidmann
Mar 28, 2012·The Journal of Reproduction and Development·Thomas E Spencer, Massimo Palmarini
Mar 17, 2012·Nature Reviews. Genetics·Cédric Feschotte, Clément Gilbert
Mar 9, 2011·Médecine sciences : M/S·Anne Dupressoir, Thierry Heidmann
Nov 26, 2014·Viruses·Adjimon Gatien LokossouBenoit Barbeau
Jan 29, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Joachim Denner
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Guillaume CornelisThierry Heidmann
Jan 19, 2016·Frontiers in Genetics·Geraldine Servant, Prescott L Deininger
Feb 16, 2013·Trends in Cell Biology·Wesley D GiffordTodd S Macfarlan
Oct 18, 2011·Current Opinion in Virology·Maulik R PatelHarmit S Malik
Aug 6, 2010·Seminars in Cancer Biology·M T RomanishD L Mager
Jun 10, 2010·American Journal of Reproductive Immunology : AJRI·Sarah G BlackThomas E Spencer
Oct 8, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuhiko ImakawaTakayuki Miyazawa
Sep 24, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Ian A WarrenJean-Nicolas Volff
Oct 8, 2015·Annual Review of Cell and Developmental Biology·Emin Maltepe, Susan J Fisher
Sep 30, 2014·Molecular Immunology·Gary EntricanDavid J Griffiths
Mar 26, 2015·Biochemical and Biophysical Research Communications·Jian ShiGengfu Xiao
Aug 11, 2012·Current Biology : CB·David Haig
Oct 2, 2012·The Veterinary Journal·Rachael E TarlintonRichard D Emes
Mar 15, 2015·Genome Biology and Evolution·Mihaela PavlicevLouis Muglia
Mar 3, 2012·Placenta·B S HolderJ D Aplin
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guillaume CornelisAnne Dupressoir
Feb 24, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M NavilleJ-N Volff
Jun 1, 2016·Database : the Journal of Biological Databases and Curation·So Nakagawa, Mahoko Ueda Takahashi

❮ Previous
Next ❯

Datasets Mentioned

BETA
GU196371

Methods Mentioned

BETA
transfection
Assay
transfections
PCR

Software Mentioned

BLAST
NJPLOT
CLUSTALW
Ensembl
BIOMOTIF
Ensembl RABBIT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
A DupressoirT Heidmann
Proceedings of the National Academy of Sciences of the United States of America
A DupressoirT Heidmann
Proceedings of the National Academy of Sciences of the United States of America
Sandra BlaiseT Heidmann
Proceedings of the National Academy of Sciences of the United States of America
Kathrin A DunlapThomas E Spencer
© 2022 Meta ULC. All rights reserved