PMID: 225444Jan 1, 1979Paper

Identification of an enteric adenovirus by immunoelectroosmophoresis (IEOP) technique

Journal of Medical Virology
P A JacobssonG Wadell

Abstract

Infantile gastroenteritis can be caused by several of the established adenovirus types. There are also adenoviruses that defy in vitro cultivation but have been recognized by electron microscopy. One isolate of these viruses, which have been designated enteric adenoviruses, has been characterized. Several of the established adenovirus types are shed in stools over long periods. The conditions for direct identification of enteric adenoviruses by immunoelectroosmophoresis (IEOP) have therefore been evaluated. Application of this technique requires highly specific reagents. However, a high prevalence of antibodies in rabbits reacting with human rotavirus was noted. For these reasons, immunoadsorbent purified antibodies were prepared. Because of the difficulty in purifying immunogens from stools, an immunization procedure characterized by immunization with adenovirus subunits bound to affinity chromatography beads was elaborated. An identification procedure for adenoviruses causing infantile gastroenteritis based a) on IEOP using group-specific and monospecific antibodies and b) on the determination of the in vitro cultivatability is suggested.

References

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Citations

Jan 1, 1986·Archives of Virology·M J Albert
Jan 1, 1993·Archives of Virology·P A ThörnerM E Johansson
Jun 1, 1982·The Journal of Hygiene·A H KiddC R Madeley
Feb 1, 1981·Journal of Clinical Pathology·A H Kidd, C R Madeley
Jan 1, 1989·Clinical Microbiology Reviews·M L Christensen
Sep 1, 1990·Baillière's Clinical Gastroenterology·I UhnooG Wadell
Jan 1, 1984·Journal of Medical Virology·A H KiddB D Schoub
Feb 1, 1985·Journal of Medical Virology·J P LeiteH G Schatzmayr
Sep 1, 1985·Journal of Medical Virology·M E JohanssonG Wadell
Jan 1, 1983·Journal of Medical Virology·J C de JongR G Firtzlaff
Jan 1, 1980·Annals of the New York Academy of Sciences·G WadellG Sundell
Dec 1, 1987·Journal of Medical Virology·M GrandienI Uhnoo
Jul 1, 1982·Journal of Clinical Microbiology·J C HierholzerH C Spencer
Jun 1, 1980·Journal of Clinical Microbiology·P HalonenE Torfason
Dec 1, 1990·Journal of Clinical Microbiology·A AllardG Wadell

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