PMID: 225444Jan 1, 1979Paper

Identification of an enteric adenovirus by immunoelectroosmophoresis (IEOP) technique

Journal of Medical Virology
P A JacobssonG Wadell


Infantile gastroenteritis can be caused by several of the established adenovirus types. There are also adenoviruses that defy in vitro cultivation but have been recognized by electron microscopy. One isolate of these viruses, which have been designated enteric adenoviruses, has been characterized. Several of the established adenovirus types are shed in stools over long periods. The conditions for direct identification of enteric adenoviruses by immunoelectroosmophoresis (IEOP) have therefore been evaluated. Application of this technique requires highly specific reagents. However, a high prevalence of antibodies in rabbits reacting with human rotavirus was noted. For these reasons, immunoadsorbent purified antibodies were prepared. Because of the difficulty in purifying immunogens from stools, an immunization procedure characterized by immunization with adenovirus subunits bound to affinity chromatography beads was elaborated. An identification procedure for adenoviruses causing infantile gastroenteritis based a) on IEOP using group-specific and monospecific antibodies and b) on the determination of the in vitro cultivatability is suggested.


May 8, 1976·Lancet·C B RodningS L Erlandsen
Nov 6, 1976·Lancet·R N SuttonP N Swift
Jan 1, 1979·Journal of Immunological Methods·M E JohanssonL Svensson
Apr 1, 1977·American Journal of Epidemiology·J P FoxM K Cooney
Jul 1, 1977·American Journal of Diseases of Children·P J MiddletonM Petric
Jan 1, 1978·Journal of Immunological Methods·M E Johansson, G Wadell
Jan 1, 1976·Journal of Immunological Methods·M E JohanssonM Grandien
May 1, 1977·Journal of Virology·G Winberg, G Wadell
Jul 1, 1969·Virology·D O WhiteJ V Maizel
Oct 1, 1969·Japanese Journal of Medical Science & Biology·Y Moritsugu
Nov 1, 1969·Journal of Virology·E Norrby, G Wadell
Mar 1, 1969·Virology·R S SaffermanT O Diener
Mar 1, 1957·Acta Paediatrica·L KJELLENA SVEDMYR

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Jan 1, 1986·Archives of Virology·M J Albert
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Jun 1, 1982·The Journal of Hygiene·A H KiddC R Madeley
Feb 1, 1981·Journal of Clinical Pathology·A H Kidd, C R Madeley
Jan 1, 1989·Clinical Microbiology Reviews·M L Christensen
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Feb 1, 1985·Journal of Medical Virology·J P LeiteH G Schatzmayr
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Jan 1, 1980·Annals of the New York Academy of Sciences·G WadellG Sundell
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