Identification of an extracellular protein of Listeria monocytogenes possibly involved in intracellular uptake by mammalian cells.

Infection and Immunity
M Kuhn, W Goebel

Abstract

Mutants of Listeria monocytogenes were recently isolated which are impaired in the synthesis of a major extracellular protein (p60). As shown in this investigation, the p60 mutants have lost the capability of invading nonprofessional phagocytic 3T6 mouse fibroblast cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of supernatant proteins of these mutants indicated that no other extracellular protein was altered in these mutants. The p60 mutants formed long cell chains which disaggregated to normal-sized single bacteria upon treatment with partially purified p60. These disaggregated bacterial cells were able to invade 3T6 cells. Physical disruption of the cell chains by ultrasonication produced similar single cells which were, however, noninvasive. Treatment of these ultrasonicated mutant cells with wild-type p60 restored their ability to invade 3T6 cells.

References

Sep 1, 1977·Analytical Biochemistry·N Y Nguyen, A Chrambach
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1988·Infection·W GoebelM Leimeister-Wächter
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P I FieldsF Heffron
Jan 1, 1987·Reviews of Infectious Diseases·G CornelisG Wauters

❮ Previous
Next ❯

Citations

Apr 26, 2000·FEMS Microbiology Letters·J H ParkB S Yoon
Aug 16, 2002·Molecular Microbiology·Laurel L Lenz, Daniel A Portnoy
Jul 23, 2003·Infection and Immunity·Nicolas AutretAlain Charbit
Oct 25, 2007·Journal of Molecular Microbiology and Biotechnology·Séverine LayecNathalie Leblond-Bourget
Dec 7, 1989·Nature·J E Donelson, A B Fulton
Jan 1, 1991·Infection·W GoebelM Wuenscher
Mar 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J McLauchlin
Nov 9, 2000·Letters in Applied Microbiology·N J RowanA A Candlish
Sep 3, 2002·FEMS Microbiology Letters·Tomoko HanawaShigeru Kamiya
Mar 20, 2001·Infection and Immunity·A J WaligoraT Karjalainen
Apr 7, 2005·Histochemistry and Cell Biology·Elisabeth EpplerPeter Groscurth
Jul 14, 2012·Bioscience, Biotechnology, and Biochemistry·Pongsak Rattanachaikunsopon, Parichat Phumkhachorn
Jan 1, 1997·Critical Reviews in Microbiology·A K Bhunia
Aug 1, 1997·Immunological Reviews·S D Fleming, P A Campbell
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Hélène Bierne, Pascale Cossart
Oct 19, 2004·Annual Review of Microbiology·Olivier DussurgetPascale Cossart
Dec 24, 2002·Infection and Immunity·Hélène Réglier-PoupetPatrick Berche
Oct 9, 2007·Electrophoresis·Sana MujahidChinling Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.