PMID: 11909835Mar 23, 2002Paper

Identification of an immunodominant drug efflux pump in Burkholderia cepacia

The Journal of Antimicrobial Chemotherapy
S M WigfieldJ P Burnie

Abstract

Burkholderia cepacia, a major pathogen amongst individuals with cystic fibrosis (CF), is intrinsically resistant to most clinically available antibiotics. We report the identification of an immunodominant antigen in CF patients infected with B. cepacia, a multidrug-resistance efflux pump called BcrA. The bcrA gene encodes a 46 kDa peptide with 14 potential alpha-helices that belongs to the major facilitator superfamily of drug transporters. A recombinant Escherichia coli strain was constructed containing the bcrA gene, which resulted in a four-fold increase in resistance to tetracycline and an eight-fold increase in resistance to nalidixic acid. These results demonstrate that the bcrA gene is part of a drug efflux system that is potentially a major contributor to the high-level antibiotic resistance observed in B. cepacia and thus a potential target for novel therapeutics.

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Citations

Jun 9, 2005·Advanced Drug Delivery Reviews·Ayush Kumar, Herbert P Schweizer
Mar 21, 2012·International Journal of Environmental Health Research·Ana MaravićMaja Pavela-Vrančić
Mar 2, 2011·Antimicrobial Agents and Chemotherapy·Tom CoenyeEshwar Mahenthiralingam
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Jan 14, 2004·Drugs·Xian-Zhi Li, Hiroshi Nikaido
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Mar 17, 2015·Expert Review of Anti-infective Therapy·Vikas GautamAmit Agarwal
Aug 8, 2021·International Journal of Molecular Sciences·Fernando Sanz-GarcíaSara Hernando-Amado

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