Dec 1, 1987

Identification of an inducible endothelial-leukocyte adhesion molecule

Proceedings of the National Academy of Sciences of the United States of America
M P BevilacquaM A Gimbrone

Abstract

The accumulation of blood leukocytes at sites of inflammation depends upon their localized adhesion to the vascular lining. We have investigated the hypothesis that this adhesive interaction involves inducible endothelial cell-surface structures that can bind leukocytes. Certain inflammatory/immune cytokines, namely interleukin 1, tumor necrosis factor, and lymphotoxin, as well as bacterial endotoxin, act on cultured human endothelial cells (HEC) in a time- and protein-synthesis-dependent fashion to increase leukocyte adhesion. We have developed two monoclonal antibodies (mAbs), H18/7 and H4/18, that identify a cell-surface antigen expressed on cytokine- and endotoxin-stimulated HEC but not on unstimulated HEC. Both mAbs immunoprecipitate the same polypeptides (major species, Mr 115,000; minor species, Mr 97,000, reduced) from biosynthetically labeled cytokine-stimulated HEC. The mediator specificity and kinetics of HEC expression of this protein(s) correlate with increased adhesiveness for leukocytes. In standardized endothelial-leukocyte adhesion assays, mAb H18/7 inhibits the adhesion of polymorphonuclear leukocytes (greater than 50%) and HL-60 cells (greater than 60%) to stimulated HEC by comparison to isotype-matched contr...Continue Reading

Mentioned in this Paper

Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Leukocyte Cell-cell Adhesion
Tumor Necrosis Factor-alpha
Natural Products
Hormone Receptors, Cell Surface
Interleukin-1
Blood Vessel
White Blood Cell Count Procedure
Immunoglobulin Isotypes

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