PMID: 6982271Sep 1, 1982Paper

Identification of an intracellular postsynaptic antigen at the frog neuromuscular junction

The Journal of Cell Biology
S J Burden

Abstract

A layer of amorphous, electron-dense material is situated at the cytoplasmic surface of the postsynaptic membrane of vertebrate neuromuscular synapses. The function of this structure is not clear, but its location suggests that it may have an important role in the formation and/or maintenance of the synapse. This paper demonstrates that a monoclonal antibody raised against antigens from Torpedo electric organ binds to an intracellular, postsynaptic protein at the frog neuromuscular synapse. Indirect immunofluorescence on frozen sections of frog muscle was used to demonstrate that the antigen is concentrated at synaptic sites in normal muscle. In denervated muscle, the antigen remains concentrated at synaptic sites, but is also present at extrasynaptic regions of denervated myofibers. The antigen cannot be labeled in intact, whole muscle, but only in whole muscle that has been permeabilized with nonionic detergents. The antibody staining pattern in Triton X-100-permeabilized whole-mounts of the frog neuromuscular synapse is arranged in elongate, arborized areas which are characteristic of the frog neuromuscular synapse. The stained areas are striated and the striations occur with a periodicity that corresponds to the regular fol...Continue Reading

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Citations

Jan 1, 1988·Pharmacology & Therapeutics·F J Barrantes
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S J Burden
Mar 1, 1986·The Journal of Cell Biology·M J Anderson
Apr 1, 1986·The Journal of Cell Biology·R J Bloch
Jan 1, 1987·The Journal of Cell Biology·L L Englander, L L Rubin
Feb 1, 1989·The Journal of Cell Biology·R J Bloch, J S Morrow
Nov 1, 1991·The Journal of Cell Biology·J E YeadonS J Burden
Apr 11, 1990·Nucleic Acids Research·D E FrailJ P Merlie
Apr 1, 1999·FEBS Letters·S Balasubramanian, R L Huganir
Jun 1, 1989·Journal of Electron Microscopy Technique·H Yorifuji, N Hirokawa
Aug 1, 1988·Archives of Disease in Childhood·A L BrunettoS J Pedler
Mar 1, 1988·The American Journal of Physiology·R J Bloch, D W Pumplin
Sep 21, 1984·Science·L F Reichardt

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