Identification of an IS6110 insertion site in plcD, the unique phospholipase C gene of Mycobacterium bovis

Journal of Medical Microbiology
Cristina Viana-NieroSylvia Cardoso Leão

Abstract

The IS6110 repetitive element is present in multiple copies in most Mycobacterium tuberculosis complex bacteria, except for Mycobacterium bovis strains, which usually contain a single copy of IS6110 located on a 1.9 kb PvuII fragment of the direct repeat region. IS6110 transposition can disrupt coding regions and is a major force of genomic variation. In a previous work it was demonstrated that phospholipase C genes are preferential loci for IS6110 transposition in M. tuberculosis clinical strains. Bacterial phospholipase C enzymes participate in pathogenic mechanisms used by different organisms, and have been implicated in intracellular survival, cytolysis and cell-to-cell spread. Four phospholipase C genes (plcA, plcB, plcC and plcD) were detected in the genomes of M. tuberculosis, Mycobacterium africanum, Mycobacterium microti and 'Mycobacterium canettii'. M. bovis and the vaccine strain M. bovis Bacillus Calmette-Guérin contain only the plcD gene. In the present work, the existence of IS6110 insertions within plcD, the unique phospholipase C gene of M. bovis, has been investigated by PCR, Southern blot hybridization and sequencing analysis. In 18 (7.3 %) of 245 isolates analysed, the plcD gene was interrupted by the inserti...Continue Reading

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Citations

May 17, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Noel H SmithKristin Kremer
Sep 29, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Noel H Smith
Sep 16, 2016·PloS One·Ricardo César Tavares CarvalhoVânia Margaret Flosi Paschoalin
Feb 9, 2012·Memórias do Instituto Oswaldo Cruz·Patrícia Martins ParreirasPhilip Noel Suffys
May 14, 2021·Frontiers in Veterinary Science·Érica Bravo SalesMarcos Bryan Heinemann

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