Identification of an mRNA element promoting rate-limiting cleavage of the polycistronic puf mRNA in Rhodobacter capsulatus by an enzyme similar to RNase E

Molecular Microbiology
J FritschG Klug

Abstract

We have identified an mRNA element that is involved in the initial cleavage of the pufBALMX mRNA species in Rhodobacter capsulatus. This endoribonuclease recognition site, the first to be identified in a bacterial species other than Escherichia coli, shows strong similarities to mRNA sequences cleaved by the endoribonuclease E in E. coli. The presence of an RNase E-like enzyme in R. capsulatus is further supported by in vitro cleavage of E. coli transcripts by R. capsulatus extracts at sites attributed to RNase E and by the cross-reaction of a polypeptide from R. capsulatus with antisera against E. coli RNase E. Our data provide evidence that mRNAs are degraded in different bacterial species by enzymes with similar recognition sequences and activities. We present a model that attributes the segmental differences in stability of the polycistronic puf transcript to a specific distribution of mRNA decay-promoting and mRNA decay-impeding elements.

References

Jan 1, 1992·Genes & Development·S A EmoryJ G Belasco
Oct 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C P EhretsmannH M Krisch
Dec 20, 1992·Journal of Molecular Biology·R S Cormack, G A Mackie
Jun 1, 1992·Journal of Bacteriology·A M Patel, S D Dunn
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G Klug
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M P Deutscher, N B Reuven
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P Babitzke, S R Kushner
Nov 20, 1986·Journal of Molecular Biology·V J CannistraroD Kennell
Nov 25, 1987·Nucleic Acids Research·C EhresmannB Ehresmann
May 1, 1989·European Journal of Biochemistry·V J Cannistraro, D Kennell
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A von GabainS N Cohen
Mar 1, 1983·European Journal of Biochemistry·M K RoyD Apirion
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B SohlbergA von Gabain
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R S CormackG A Mackie

❮ Previous
Next ❯

Citations

Jan 1, 1996·Biochimie·L C RaynalA J Carpousis
Nov 20, 2001·Nucleic Acids Research·S JägerG Klug
Jul 21, 2004·Journal of Molecular Microbiology and Biotechnology·Stephanie JägerGabriele Klug
Sep 26, 2013·Cellular and Molecular Life Sciences : CMLS·Soumaya LaalamiHarald Putzer
May 2, 1997·Journal of Molecular Biology·C CondonM Grunberg-Manago
Mar 10, 2001·Environmental Microbiology·K Takayama, S Kjelleberg
Mar 1, 1997·Molecular Microbiology·S N Cohen, K J McDowall
Mar 3, 2004·Microbiology·Stephanie JägerGabriele Klug
Jun 26, 1999·Molecular Microbiology·G HomuthW Schumann
Feb 12, 2005·The Journal of Biological Chemistry·Rajyaguru Ichchhashankar PurusharthGabriele Klug
Jun 17, 1999·FEMS Microbiology Reviews·R Rauhut, G Klug
Mar 21, 2007·The Journal of Biological Chemistry·Steffen Wagner, Gabriele Klug
Aug 8, 1998·Journal of Bacteriology·G C PaoliF R Tabita
Feb 1, 1997·Journal of Bacteriology·B FranzettiA von Gabain

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.