Identification of an octamer-binding site in the mouse kappa light-chain immunoglobulin enhancer.

Molecular and Cellular Biology
R A Currie, R G Roeder

Abstract

A 215-base-pair (bp) region of the mouse MOPC 41 kappa light-chain immunoglobulin gene enhancer has been analyzed for specific binding of lymphoid and nonlymphoid nuclear factors. Mobility shift assays with a series of overlapping DNA fragments have mapped DNA-binding sites for three unique factors. The B-cell-specific (OTF-2) and ubiquitous (OTF-1) octamer-binding transcription factors specifically bound to a site centered about 136 bp 5' of the nuclear factor NF-kappa B site. A third specific factor, NF-kappa E, bound to a site that was about 75 bp 5' of the NF-kappa B site and within a region important for enhancer function. This novel factor was found in both mature B and HeLa cell nuclei. B-cell OTF-2, B-cell OTF-1, and HeLa OTF-1 bound to the kappa enhancer and kappa promoter octamer sites with similar affinities despite a 2-bp difference in the kappa enhancer octamer sequence. However, DNase I footprint analyses indicated that affinity-purified OTF-2 bound both to the enhancer OTF site and, surprisingly, to 80 bp of A + T-rich flanking sequence. Moreover, methylation interference studies demonstrated distinct differences in OTF interactions between the consensus octamer in the kappa promoter and the nonconsensus octamer ...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Jul 24, 1986·Nature·J V GarciaC Queen
Feb 1, 1986·Immunological Reviews·F W AltG D Yancopoulos
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Mizushima-Sugano, R G Roeder
Dec 1, 1988·Genes & Development·R G ClercP A Sharp
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H L Sive, R G Roeder
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L DaileyN Heintz
Jun 1, 1984·Molecular and Cellular Biology·C Queen, J Stafford
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T G ParslowD K Granner
Jan 1, 1983·Annual Review of Immunology·R Wall, M Kuehl
Jan 1, 1981·Immunological Reviews·T HonjoN Ishida
Dec 21, 1981·Nucleic Acids Research·E L Mather, R P Perry
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·U Siebenlist, W Gilbert

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Citations

Jul 1, 1995·Immunobiology·T WirthH König
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I CoquilleauM Goodhardt
Mar 1, 1991·Molecular and Cellular Biology·J W PierceD Baltimore
Jul 1, 1990·Molecular and Cellular Biology·K Nelms, B Van Ness

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