Identification of an OsPR10a promoter region responsive to salicylic acid

Planta
Seon-Hee HwangDuk-Ju Hwang

Abstract

Orysa sativa pathogenesis-related protein 10a (OsPR10a) was induced by pathogens, salicylic acid (SA), jasmonic acid (JA), ethephon, abscisic acid (ABA), and NaCl. We tried to analyze the OsPR10a promoter to investigate the transcriptional regulation of OsPR10a by SA. We demonstrated the inducibility of OsPR10a promoter by SA using transgenic Arabidopsis carrying OsPR10a:GFP as well as by transient expression assays in rice. To further identify the promoter region responsible for its induction by SA, four different deletions of the OsPR10a promoter were made, and their activities were measured by transient assays. The construct containing 687-bp OsPR10a promoter from its start codon exhibited a six-fold increase of induction compared to the control in response to SA. Mutation in the W-box like element 1 (WLE 1) between 687 and 637-bp from TGACA to TGAAA completely abolished induction of the OsPR10a promoter by SA, indicating that the WLE 1 between -687 and -637 of OsPR10a promoter is important in SA-mediated OsPR10a expression. We show for the first time that the W-box like element plays a role in SA mediated PR gene expression.

References

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Citations

Jul 6, 2012·Molecular Biotechnology·Parinita AgarwalJitendra Chikara
Jul 21, 2011·Molecular Plant-microbe Interactions : MPMI·Dong-Hai PengMing Sun
Apr 24, 2009·Plant Physiology·Yinghong PanMary A Schuler
Apr 30, 2009·Annual Review of Phytopathology·A Corina VlotDaniel F Klessig
Feb 18, 2016·Scientific Reports·Sung-Il KimHak Soo Seo
Oct 13, 2015·Journal of Insect Physiology·Jagadish S BenturSuresh Nair
Jul 19, 2011·Plant Science : an International Journal of Experimental Plant Biology·Seon-Hee HwangDuk-Ju Hwang
Jan 29, 2014·Plant Science : an International Journal of Experimental Plant Biology·Carlos M Hernandez-Garcia, John J Finer
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yan ZhaoGuangcun He
Apr 10, 2020·Scientific Reports·Courtney A HollenderChris Dardick
Dec 18, 2013·Molecular Biology Reports·Parinita Agarwal, Pradeep K Agarwal

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Datasets Mentioned

BETA
AL845342

Methods Mentioned

BETA
PCR
PCRs
transgenic
fluorescence microscopy

Software Mentioned

PLAnt
PLACE

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