PMID: 8421684Jan 15, 1993Paper

Identification of an RNA binding site for human thymidylate synthase

Proceedings of the National Academy of Sciences of the United States of America
Edward ChuCarmen J Allegra

Abstract

Previous studies from this laboratory have shown that human TS mRNA translation is regulated by its protein product in a negative autoregulatory manner. In this paper, we identify an RNA binding site for TS protein located within the first 188 nt of TS RNA. A 36-nt RNA sequence contained within this 188-nt fragment, corresponding to nt 75-110 and including the translational initiation site, binds TS protein with an affinity similar to that of both the full-length and the 188-nt TS RNA sequences. Variant RNAs with either a deletion or a mutation at the translational initiation region are unable to compete for TS protein binding. UV crosslinking studies reveal that an RNA fragment of approximately 36 nt is protected from RNase T1 digestion by TS protein binding. A second TS protein-binding site is localized within the protein-coding region corresponding to nt 434-634. These findings demonstrate a specific interaction between human TS protein and its TS RNA and identify an RNA binding site that includes the translational initiation site.

References

Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E ChuC J Allegra
May 11, 1991·Nucleic Acids Research·K JolliffL F Johnson
Nov 17, 1989·Science·R D Klausner, J B Harford
Mar 25, 1985·Nucleic Acids Research·K TakeishiT Seno
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E A Leibold, H N Munro
Mar 24, 1987·Biochemistry·P J RomaniukO C Uhlenbeck
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A Bernardi, P F Spahr
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M BelfortF Maley
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies

❮ Previous
Next ❯

Citations

Mar 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E Chu, C J Allegra
Apr 1, 1994·Journal of Cellular Biochemistry·A B Pardee
Jan 1, 1996·Stem Cells·E ChuC J Allegra
Jun 1, 1995·Journal of Molecular Evolution·N C Kyrpides, C A Ouzounis
May 1, 1994·Molecular Biology Reports·N K Gray, M W Hentze
Jan 12, 2007·Applied Microbiology and Biotechnology·Anatoly ChernyshevAmnon Kohen
Aug 4, 2011·Amino Acids·Oscar Perez-Leal, Salim Merali
Sep 20, 2007·Science in China. Series C, Life Sciences·Song YanXiuKun Lin
Jan 1, 1996·Advances in Enzyme Regulation·E Chu, C J Allegra
Jan 1, 1994·Biochimie·N Standart, R J Jackson
Jan 17, 2004·Biochimica Et Biophysica Acta·Sondra H BergerLukasz Lebioda
Jun 29, 2013·Biochemical and Biophysical Research Communications·Oscar Perez-LealSalim Merali
Apr 16, 1998·Pharmacology & Therapeutics·W R Montfort, A Weichsel
Aug 1, 1996·Current Opinion in Genetics & Development·P M Macdonald, C A Smibert
Apr 29, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G J PetersH M Pinedo
Jul 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniela CardinaleM Paola Costi
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·F CarrierA J Fornace
Oct 17, 2007·Journal of Biomolecular Structure & Dynamics·Abul HasnatJulie Andrews
Nov 25, 1993·Nucleic Acids Research·S D ElzingaL A Grivell
Jun 25, 1995·Nucleic Acids Research·O P de Melo NetoC Martins de Sa
Apr 25, 2000·Nucleic Acids Research·J Cho, R R Rando
Dec 8, 2009·Nucleic Acids Research·Glen MeadesGrover L Waldrop
Feb 21, 2013·Nucleic Acids Research·Divita GargRebecca C Wade
Jan 20, 2011·Acta Crystallographica. Section D, Biological Crystallography·Sergey DibrovThomas Hermann
Mar 15, 1996·European Journal of Biochemistry·V M Pain
Apr 1, 1996·European Journal of Biochemistry·J Bag, J Wu
Feb 23, 2012·Molecular and Cellular Biology·Oscar Perez-LealSalim Merali
Feb 7, 2004·American Journal of Respiratory Cell and Molecular Biology·Sreerama ShettySteven Idell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.