Identification of an uncoupling mutation affecting the b subunit of F1F0 ATP synthase in Escherichia coli

FEBS Letters
T L CavistonB D Cain

Abstract

A specific b subunit arginine, b(Arg-36) in Escherichia coli, displays evolutionary conservation among bacterial F1F0 ATP synthases. Site-directed mutagenesis was used to generate a collection of mutations affecting b(Arg-36). The phenotype differed depending upon the substitution, and the b(Arg-36-Glu) and b(Arg-36-Ile) substitutions virtually abolished enzyme function. Although the total amounts of F1F0 ATP synthase present in the membranes prepared from mutant strains were reduced, the primary effect of the b(Arg-36) substitutions was on the activities of the intact enzyme complexes. The most interesting result was that the b(Arg-36-Glu) substitution results in the uncoupling of a functional F0 from F1 ATP hydrolysis activity.

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Citations

May 21, 2005·Journal of Bioenergetics and Biomembranes·Deepa BhattBrian D Cain
Jan 22, 2008·Journal of Bioenergetics and Biomembranes·Amanda K WelchBrian D Cain
Apr 17, 2009·The Journal of Biological Chemistry·Shane B ClaggettBrian D Cain
Sep 6, 2005·The Journal of Biological Chemistry·Mali LiuPatricia M Kane
May 29, 2007·Journal of Bacteriology·Shane B ClaggettBrian D Cain
Feb 20, 2003·Journal of Bacteriology·Wendi L Kuhnert, Robert G Quivey
Aug 22, 2006·Biochemical and Biophysical Research Communications·Yeon T ChungDale A Webster
Jun 19, 2007·Biochimica Et Biophysica Acta·Guadalupe GuerraCarolyn W Slayman
Oct 16, 1999·The Journal of Biological Chemistry·M RevingtonS D Dunn
Oct 17, 1998·The Journal of Biological Chemistry·P L SorgenB D Cain
Sep 10, 2004·The Journal of Antimicrobial Chemotherapy·Nicole M ParrishJames D Dick
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Mark S Dillingham, Stephen C Kowalczykowski
Dec 14, 1999·The Journal of Biological Chemistry·P L SorgenB D Cain
May 26, 2004·The Journal of Biological Chemistry·Tammy Bohannon Grabar, Brian D Cain
Apr 5, 2000·The Journal of Biological Chemistry·D T McLachlinS D Dunn
Jun 6, 2000·Biochimica Et Biophysica Acta·S D DunnM Revington
Apr 1, 2007·EcoSal Plus·Steven B Vik
Oct 10, 2006·Journal of Molecular Biology·Paul A Del RizzoStanley D Dunn

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