Identification of androgen-binding protein from testis cytosol and sertoli cell culture medium of the cynomolgus monkey, Macaca fascicularis

Endocrinology
H S KeepingP Troen

Abstract

The biochemical and immunological properties of monkey androgen-binding protein (mABP) from the cynomolgus monkey, Macaca fascicularis, have been examined in testis cytosol and medium of primary Sertoli cell-enriched cultures. mABP in testis tissue was separated from the serum protein testosterone-estradiol-binding globulin (mTeBG) by affinity chromatography on Concanavalin A-Sepharose (Con A). mTeBG from serum extracts was completely retained by the lectin and could be displaced with buffer containing alpha-methyl-D-glucoside. In contrast, mABP from testis extracts either did not interact with the Con A and appeared in the void volume or was partially retained by the column and could be eluted with buffer alone. A third component retained by the Con A may represent mTeBG contamination, a form of mABP which binds to Con A, or both. The specific 5 alpha-dihydrotestosterone (DHT)-binding activity in the void volume of the Con A column was designated mABP and was further studied. [3H]DHT binding to mABP was saturable and of limited capacity (0.163 +/- 19 pmol/mg protein). Scatchard analysis of the data was consistent with a single class of binding sites with an apparent dissociation constant (Kd) at 4 C of 2.6 +/- 0.2 X 10(-9) M. ...Continue Reading

Citations

May 1, 1986·Journal of Steroid Biochemistry·S J WintersP Troen
May 1, 1989·Molecular and Cellular Endocrinology·Y M WangD R Joseph
Jan 1, 1991·Annals of the New York Academy of Sciences·A GerardH Gerard
Apr 1, 1989·The Journal of Experimental Zoology·S Singh, G V Callard

Related Concepts

Androgen Binding Protein
Carrier Proteins
Chromatography, Affinity
Cytoplasmic Matrix
SDS-PAGE
Fluorometry
Macaca fascicularis
Structure of Sertoli Cell
Dihydroepitestosterone
Substrate Specificity

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