Identification of angiotensinogen and complement C3dg as novel proteins binding the proform of eosinophil major basic protein in human pregnancy serum and plasma.

The Journal of Biological Chemistry
Claus OxvigL Sottrup-Jensen

Abstract

In sera from pregnant women, pregnancy-associated plasma protein-A (PAPP-A) circulates as a disulfide-bound complex (approximately 474 kDa) with the proform of eosinophil major basic protein (proMBP) (Oxvig, C., Sand, O., Kristensen, T., Gleich, G. J., and Sottrup-Jensen, L. (1993) J. Biol. Chem. 268, 12243-12246). We have produced monoclonal antibodies (mAbs) against the PAPP-A.proMBP complex and established a radioimmunoassay utilizing a mAb recognizing the PAPP-A subunit. Surprisingly, serum levels of proMBP exceed those of PAPP-A four to 10-fold on a molar basis throughout pregnancy. This result prompted an investigation of the status of proMBP in pregnancy. Using a proMBP-specific mAb two novel proMBP complexes have been isolated by chromatographic techniques. Based on sequence analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and reaction with specific antibodies, one is shown to be a 2:2 disulfide-bound complex (approximately 200 kDa) between proMBP and angiotensinogen. The other is a 2:2:2 complex (approximately 300 kDa) between proMBP, angiotensinogen, and complement C3dg. Circulating proMBP in pregnancy is thus present in three types of complexes. These results suggest that specific interactions bet...Continue Reading

References

Jul 16, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·P BischofA Klopper
Mar 1, 1976·The Journal of Clinical Investigation·G J GleichJ E Maldonado
Nov 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·D B GordonV N Dodd
Dec 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B E SundströmT I Stigbrand
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M H de Bruijn, G H Fey
Jun 1, 1985·The Journal of Clinical Investigation·D J CampbellP Corvol
Oct 1, 1988·The Journal of Experimental Medicine·R L BarkerL R Pease
Dec 1, 1988·The Journal of Experimental Medicine·M McGroganJ Gabay
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T L WasmoenG J Gleich
Jan 1, 1986·Advances in Immunology·G J Gleich, C R Adolphson
May 1, 1986·The Journal of Endocrinology·H J Tetlow, F Broughton Pipkin
Jul 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·D A TewksburyR A Dart
Jan 15, 1974·American Journal of Obstetrics and Gynecology·T M LinS Gall
Jun 1, 1973·The Journal of Experimental Medicine·G J GleichJ E Maldonado
Jun 1, 1980·Molecular Immunology·M UotilaE Ruoslahti
Apr 15, 1981·American Journal of Obstetrics and Gynecology·J FolkersenJ G Westergaard
Jan 1, 1983·Archives of Gynecology·J G WestergaardJ G Grudzinskas
Dec 1, 1982·American Journal of Reproductive Immunology : AJRI·B TeisnerJ G Grudzinskas
Mar 1, 1981·The Journal of Clinical Investigation·D L WassomG J Gleich
Jul 5, 1993·Journal of Molecular Biology·L MoureyD Moras
Jan 2, 1993·FEBS Letters·K Dolmer, L Sottrup-Jensen
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

❮ Previous
Next ❯

Citations

Dec 1, 1996·Protein Science : a Publication of the Protein Society·E StratikosS P Becerra
Sep 12, 2000·Current Hypertension Reports·X JeunemaitreP Corvol
Oct 29, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·Q P QinB Nørgaard-Pedersen
Dec 26, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Patrick DumpsPaul Bischof
Oct 6, 2001·The New England Journal of Medicine·A Bayes-GenisR S Schwartz
Jul 1, 2005·Future Cardiology·Omar F Laterza
Sep 17, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Omar F LaterzaGary B Green
Feb 13, 2001·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·D CheliusE M Spencer
Aug 15, 2000·Placenta·A H NielsenK Poulsen
Mar 23, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Qiu-Ping QinKim Pettersson
Jan 29, 2011·Biology of Reproduction·Kathrin Weyer, Simon Glerup
Nov 20, 2004·Obstetrics and Gynecology Clinics of North America·Engin Oral, Mehmet R Genç
Oct 16, 1999·Prenatal Diagnosis·M ChristiansenB Norgaard-Pedersen
Jul 1, 2008·American Journal of Rhinology·Ender GüçlüErol Egeli
Oct 23, 1997·Endocrine Reviews·P Corvol, X Jeunemaitre
Nov 11, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Roberto RomeroLami Yeo
Oct 4, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Eugenie R Lumbers, Kirsty G Pringle
Jun 15, 2007·Advances in Therapy·Abdurrahman CoskunEmel Kurt
Sep 14, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Lasse Bach SteffensenClaus Oxvig

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.