Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry

Journal of Microbiological Methods
Peter KuhnertJoachim Frey

Abstract

Species of the family Pasteurellaceae play an important role as primary or opportunistic animal pathogens. In veterinary diagnostic laboratories identification of this group of bacteria is mainly done by phenotypic assays while genetic identification based on housekeeping genes is mostly used for research and particularly important diagnostic samples. MALDI-TOF MS seems to represent a promising alternative to the currently practiced cumbersome, phenotypic diagnostics carried out in many veterinary diagnostic laboratories. We therefore assessed its application for animal associated members of the family Pasteurellaceae. The Bruker Biotyper 3.0 database was complemented with reference spectra of clinically relevant as well as commensal animal Pasteurellaceae species using generally five strains per species or subspecies and tested for its diagnostic potential with additional, well characterized field isolates. About 250 strains comprising 15 genera and more than 40 species and subspecies were included in the study, covering most representatives of the family. A high discrimination at the genus and species level was observed. Problematic discrimination was only observed with some closely related species and subspecies. MALDI-TOF M...Continue Reading

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Citations

Sep 11, 2013·Systematic and Applied Microbiology·Joerg JoresJoachim Frey
Apr 5, 2014·Journal of Pharmaceutical and Biomedical Analysis·Miguel Angel Merlos RodrigoRene Kizek
Feb 11, 2014·Antonie van Leeuwenhoek·Bożena M KorczakPeter Kuhnert
Aug 13, 2015·Research in Veterinary Science·Luke P RandallAdrian M Whatmore
Mar 16, 2013·Rapid Communications in Mass Spectrometry : RCM·Steffen WeidnerBernd Enthaler
Jan 31, 2015·International Journal of Systematic and Evolutionary Microbiology·Peter KuhnertBożena M Korczak
Aug 25, 2015·Frontiers in Microbiology·Neelja SinghalJugsharan S Virdi
Jul 28, 2013·Diagnostic Microbiology and Infectious Disease·Salah ZangenahVolkan Ozenci
May 8, 2014·Avian Pathology : Journal of the W.V.P.A·Merima AlispahicClaudia Hess
Aug 6, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Luisa Z MorenoAndrea M Moreno
Jan 11, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Carlos E C MatajiraAndrea M Moreno
Mar 23, 2017·Tropical Animal Health and Production·Maria Gabriela Xavier OliveiraTerezinha Knöbl
Jul 24, 2015·Journal of Clinical Microbiology·Jillian S Y LauTony M Korman
Jan 23, 2019·The Veterinary Quarterly·Yamê Miniero DaviesTerezinha Knöbl
Jan 31, 2020·International Journal of Systematic and Evolutionary Microbiology·Peter KuhnertJoerg Jores
Apr 18, 2020·Microbiology·Virginia HillRicardo A R Machado
Apr 26, 2016·The Journal of Veterinary Medical Science·Andrzej PuchalskiAndrzej Wernicki
Oct 18, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Jean-Philippe LavigneAlbert Sotto
Jul 12, 2019·The Journal of Veterinary Medical Science·Eri Uchida-FujiiToshio Nukada
Dec 19, 2018·Emerging Infectious Diseases·Holly Angela SinclairGraeme R Nimmo
Sep 24, 2019·Journal of Veterinary Dentistry·Špela SrečnikAna Nemec
Jan 21, 2021·International Journal of Systematic and Evolutionary Microbiology·Peter KuhnertMagne Bisgaard
Apr 1, 2021·Brazilian Journal of Biology = Revista Brasleira De Biologia·Y M DaviesT Knöbl
May 5, 2021·Journal of Veterinary Internal Medicine·Flavie PayetteMathilde Leclere
Jul 16, 2021·International Journal of Systematic and Evolutionary Microbiology·Henrik ChristensenMagne Bisgaard
Nov 9, 2012·Analytical Chemistry·Vladimir HavlicekKevin A Schug

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