PMID: 9178613Jun 1, 1997Paper

Identification of anion-selective channels in the basolateral membrane of mitochondria-rich epithelial cells

The Journal of Membrane Biology
N J Willumsen, E H Larsen

Abstract

Epithelial cells of toad (Bufo bufo) skin were isolated by treatments of the epidermis with collagenase and trypsin. Cl- channels in the basolateral membrane from soma or neck of mitochondria-rich cells were studied in cell-attached and excised inside-out configurations. Of a total of 87 sealed patches only 28 (32%) were electrically active, and in these we identified four different types of Cl- channels. The two major populations constituted Ohmic Cl- channels with limiting conductance (gamma125/125) of 10 pS and 30 pS, respectively. A much rarer 150 pS Ohmic Cl- channel was also characterized. From i/V relationships of individual channels the following Goldman-Hodgkin-Katz permeabilities were calculated, 2.2 (+/-0.1) x 10(-14), 5.7 (+/-0.7) x 10(-14), and 32 (+/-2) x 10(-14) cm3/sec, for the 10, 30 and 150 pS Cl- channels, respectively. The 30 pS channel was activated by hyperpolarization. The gating kinetics of the 150 pS channel was complex with burstlike closures within openings of long duration. The fourth type of Cl- channel was studied in patches generating 'noisy currents' with no discrete single-channel events, but with vanishing fluctuations at pipette potentials near ECl. Noise analysis revealed a power spectrum wit...Continue Reading

Citations

Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J AmstrupE H Larse
Apr 27, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·W Nagel, U Katz
Feb 28, 2004·Public Health Nursing·Cheryl Delgado
Nov 8, 2002·Biochimica Et Biophysica Acta·Niels J WillumsenE Hviid Larsen
Jan 20, 2004·Biochimica Et Biophysica Acta·Lars Jørn JensenErik Hviid Larsen

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