Identification of antibodies against extracellular matrix proteins in human osteoarthritis

Biochemical and Biophysical Research Communications
Johannes RuthardAndreas R Klatt

Abstract

We investigated the presence of autoantibodies against the extracellular matrix proteins thrombospondin-4 (TSP-4), cartilage oligomeric matrix protein (COMP), C-type lectin domain family 3 member A (CLEC3A), collagen II, collagen VI, matrilin-3, and fibrillin-2 in the serum of osteoarthritis (OA) patients. We compared those results with the presence of such antibodies in rheumatoid arthritis (RA) patients and in healthy donors (HD). Our study examines whether antibodies against extracellular proteins can be used as potential biomarkers to support the clinical diagnosis of OA. 10 OA, 10 RA patients and 10 HD were enrolled in this explorative cross-sectional study. SDS-PAGE and immunoblot were used to investigate the presence of antibodies against extracellular matrix proteins. The serum of 5/10 OA patients but 0/10 HD exhibited TSP-4 IgG isotype antibodies (P = 0.033). The serum of 8/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4 or COMP (P = 0.005). The serum of 9/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4, COMP or CLEC3A (P = 0.005). We found strong evidence for the presence of IgG isotype autoantibodies against the cartilage extracellular matrix proteins TSP-...Continue Reading

Citations

Sep 1, 2019·Annals of the Rheumatic Diseases·María Camacho-EncinaFrancisco J Blanco
May 21, 2020·Proteins·Christoph ÖhlknechtChris Oostenbrink
Jan 24, 2019·International Journal of Molecular Sciences·Kathrin MalyFrank Zaucke
Dec 31, 2019·Osteoarthritis and Cartilage·W E van Spil, I A Szilagyi
May 6, 2021·Journal of Clinical Medicine·Susanne GrässelHenning Madry

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