Identification of antibody classes and Fc receptors responsible for phagocytosis of Trypanosoma musculi by mouse macrophages.

Infection and Immunity
P VincendeauS Daulouède

Abstract

The phagocytosis of Trypanosoma musculi by macrophages in the presence of specific antibodies was investigated. In 14-day-infected mice, opsonic antibodies were detected in serum, and phagocytosis of parasites by peritoneal macrophages was observed. The mechanism of T. musculi phagocytosis was analyzed. The binding of trypanosomes to peritoneal macrophages and J774 cells was observed in the presence of serum from hyperimmune mice and from mice infected 14 or 28 days earlier, but not in the presence of control mouse serum or sera from 7-day-infected mice. Binding was partially inhibited by mouse monoclonal immunoglobulins G1 (IgG1) or IgG2a and almost completely inhibited by a mixture of both. Binding was also partially inhibited by the anti-Fc gamma 1/gamma 2b receptor monoclonal antibody 2.4G2. Binding of T. musculi was also induced by fractions of serum from 28-day-infected mice obtained by protein A-Sepharose chromatography. Only the IgG1-rich fraction eluted at pH 6.0 and the IgG2a-rich fraction eluted at pH 4.5 promoted binding which could be almost completely inhibited by monoclonal IgG1 and IgG2a. These data indicate that IgG1 and IgG2a anti-T. musculi antibodies are responsible for the phagocytosis of T. musculi by mous...Continue Reading

References

Feb 1, 1978·The Australian Journal of Experimental Biology and Medical Science·A FerranteP C Reade
Apr 1, 1978·The Australian Journal of Experimental Biology and Medical Science·A Ferrante, C R Jenkin
Feb 1, 1979·Cellular Immunology·A Ferrante, C R Jenkin
May 1, 1977·Infection and Immunity·J A Jarvinen, A P Dalmasso
Apr 1, 1975·International Journal for Parasitology·P ViensW H Lumsden
Aug 1, 1974·Experimental Parasitology·T TakayanagiG L Enriquez
Apr 1, 1984·Infection and Immunity·F D VargasP A Kongshavn
Dec 1, 1983·International Journal for Parasitology·P ViensP A Kongshavn
Jun 1, 1984·Infection and Immunity·D S Wechsler, P A Kongshavn
Jan 1, 1983·The American Journal of Tropical Medicine and Hygiene·H C GreenblattM Aikawa
Dec 1, 1982·Mechanisms of Ageing and Development·J W Albright, J F Albright
Nov 1, 1981·Infection and Immunity·P VincendeauR Pautrizel

❮ Previous
Next ❯

Citations

Jan 1, 1992·Immunologic Research·M W Fanger, D V Erbe
Oct 22, 2013·Parasite : Journal De La Société Française De Parasitologie·Mariette DethouaPhilippe Vincendeau
Apr 1, 1989·Parasitology·P VincendeauB Veyret
Jul 26, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Romaric Nzoumbou-BokoPhilippe Vincendeau
Sep 1, 1991·Infection and Immunity·K T ShawP A Kongshavn
Sep 1, 1988·Infection and Immunity·D S Wechsler, P A Kongshavn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.