Identification of antigen Ag43 in uropathogenic Escherichia coli Dr+ strains and defining its role in the pathogenesis of urinary tract infections

Microbiology
Beata M Zalewska-PiatekJózef Kur

Abstract

Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are amongst the most common bacterial infectious diseases in the developed world. The urovirulence of UPEC is mainly associated with the surface-exposed fimbrial adhesins and adhesins of the autotransporter (AT) family. The best studied of these proteins is antigen Ag43 mediating cell aggregation, adhesion and biofilm development as the causes of chronic UTIs. The E. coli IH11128 Dr(+) (dra (+)) strain of the Dr/Afa(+) family of adhesins possesses two major surface-exposed virulence factors: Dr fimbrial polyadhesin and DraD protein (fimbrial tip subunit or protein component of the adhesive sheath). Here, we identified for the first time, to our knowledge, the agn43 gene encoding Ag43 in the WT clinical isolate of UPEC Dr(+) as a new virulence factor not yet tested. We also found that Dr fimbrial expression, which like Ag43 is under the control of a phase-variable mechanism, did not exclude Ag43 surface presentation. However, the presence of Dr fimbriae supported by other structures on the cell surface caused a physical neutralization of Ag43-mediated autoaggregation during in vitro growth. The fimbrial bundling further increased the distance between...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P Owen, H R Kaback
Sep 1, 1991·Journal of Bacteriology·I C BlomfieldB I Eisenstein
Mar 1, 1990·American Journal of Public Health·B Foxman
Dec 1, 1987·Infection and Immunity·B NowickiS I Hull
Feb 1, 1980·Journal of Bacteriology·B Diderichsen
Dec 1, 1996·FEMS Immunology and Medical Microbiology·P OwenI Henderson
Sep 5, 1997·Science·F R BlattnerY Shao
Nov 14, 1997·American Journal of Reproductive Immunology : AJRI·B NowickiC Yallampalli
Aug 10, 1999·Journal of Bacteriology·H HasmanP Klemm
Oct 24, 2000·International Journal of Medical Microbiology : IJMM·P Klemm, M A Schembri
Dec 15, 2000·Trends in Microbiology·I R HendersonJ P Nataro
Feb 17, 2001·Infection and Immunity·I R Henderson, J P Nataro
Apr 11, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L FangC Yallampalli
Jun 19, 2001·The EMBO Journal·M A Schembri, P Klemm
Mar 16, 2002·Infection and Immunity·Cristina P Van LoySteve L Moseley
Apr 3, 2002·Journal of Medical Microbiology·Berit AdamL Julia Douglas
Jul 13, 2002·The American Journal of Medicine·Betsy Foxman
Jul 24, 2002·International Journal of Antimicrobial Agents·T A OelschlaegerJ Hacker
Dec 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·R A WelchF R Blattner
Feb 5, 2003·The American Journal of Medicine
Mar 27, 2003·Molecular Microbiology·Mark A SchembriPer Klemm
Jul 5, 2003·Science·Gregory G AndersonScott J Hultgren
Jul 10, 2003·Infectious Disease Clinics of North America·Betsy Foxman, Patricia Brown
Jan 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sheryl S JusticeScott J Hultgren
Aug 26, 2004·Molecular Cell·Kirstine L AndersonStephen Matthews

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Citations

Mar 1, 2018·AIMS Microbiology·Thomas TrunkJack C Leo
May 7, 2021·Frontiers in Cellular and Infection Microbiology·Sofía V SánchezJavier O Morales

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