Identification of Antimicrobial Peptides from the Microalgae Tetraselmis suecica (Kylin) Butcher and Bactericidal Activity Improvement

Marine Drugs
Fanny GuzmánVerónica Rojas

Abstract

The outburst of microbial resistance to antibiotics creates the need for new sources of active compounds for the treatment of pathogenic microorganisms. Marine microalgae are of particular interest in this context because they have developed tolerance and defense strategies to resist the exposure to pathogenic bacteria, viruses, and fungi in the aquatic environment. Although antimicrobial activities have been reported for some microalgae, natural algal bioactive peptides have not been described yet. In this work, acid extracts from the microalga Tetraselmis suecica with antibacterial activity were analyzed, and de novo sequences of peptides were determined. Synthetic peptides and their alanine and lysine analogs allowed identifying key residues and increasing their antibacterial activity. Additionally, it was determined that the localization of positive charges within the peptide sequence influences the secondary structure with tendency to form an alpha helical structure.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1966·Canadian Journal of Microbiology·D C DuffN J Antia
Jul 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·G A WeissS S Sidhu
Aug 2, 2001·Current Opinion in Chemical Biology·K L Morrison, G A Weiss
Apr 1, 1962·Canadian Journal of Microbiology·R R GUILLARD, J H RYTHER
Oct 14, 2004·Revista médica de Chile·Felipe C Cabello
Oct 13, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·R MahalakshmiP Balaram
Nov 2, 2005·Chembiochem : a European Journal of Chemical Biology·Radhakrishnan MahalakshmiPadmanabhan Balaram
Jan 9, 2007·Biochemical and Biophysical Research Communications·César SeguraVictor Lemeshko
Apr 5, 2007·Nature Protocols·Hermann Schägger
Apr 5, 2007·Nature Protocols·Norma J Greenfield
Oct 15, 2008·Antimicrobial Agents and Chemotherapy·Hiromi Sato, Jimmy B Feix
Aug 19, 2009·The Journal of Biological Chemistry·Jennifer R Mastroianni, André J Ouellette
Nov 18, 2009·Journal of Agricultural and Food Chemistry·I-Chuan SheihPeng-Hsiang Lin
Mar 9, 2010·Nature Reviews. Microbiology·Dan I Andersson, Diarmaid Hughes
Aug 26, 2010·Regulatory Peptides·Sung-Hee LeeHyung-Sik Won
Apr 1, 2011·Biotechnology Progress·A Catarina GuedesF Xavier Malcata
Aug 25, 2012·International Journal of Biomaterials·Siti SunarintyasAlva Edy Tontowi
Oct 18, 2012·Current Pharmaceutical Biotechnology·Virginie MimouniBenoît Schoefs
Mar 6, 2015·Frontiers in Microbiology·Xinheng YuWeiwen Zhang
Mar 31, 2015·Developmental and Comparative Immunology·Paulina SchmittLuis Mercado
May 7, 2016·International Journal of Molecular Sciences·Shuai WangShiyan Qiao
Aug 6, 2016·Protein and Peptide Letters·Federico Ruiz-RuizHafiz M N Iqbal
Apr 28, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Claudio Andrés ÁlvarezFanny Guzmán
Sep 29, 2018·Advances in Microbial Physiology·Daniela GiordanoCinzia Verde
Oct 27, 2018·Journal of the Science of Food and Agriculture·Arturo IntiquillaBlanca Hernández-Ledesma
Aug 2, 2019·Food Science & Nutrition·Lhumen A TejanoYu-Wei Chang

❮ Previous
Next ❯

Citations

Apr 5, 2020·International Journal of Molecular Sciences·Elizabeth Ruiz-MárvezConcepción J Puerta
Dec 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Verónica RojasFanny Guzmán
Mar 23, 2021·Frontiers in Pharmacology·Umme Tamanna Ferdous, Zetty Norhana Balia Yusof
May 7, 2021·The ISME Journal·Conny SjöqvistAnders F Andersson
Jun 5, 2021·Physiologia Plantarum·Kari SkjånesHanne Skomedal
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fanny GuzmánConstanza Cárdenas
Oct 8, 2021·Environmental Science and Pollution Research International·Rahul Kumar GoswamiPradeep Verma

❮ Previous
Next ❯

Methods Mentioned

BETA
circular dichroism
flow cytometry
Protein Assay

Software Mentioned

FlexAnalysis
Blast
CytExpert
Graphpad Prism
PEPFOLD3 Peptide Structure Prediction
GraphPad
Spectra Manager

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.