PMID: 8963389Dec 1, 1995Paper

Identification of apoptotic hepatocytes in situ in rat liver after lead nitrate administration

Journal of Gastroenterology
T NakajimaT Okanoue

Abstract

Apoptosis plays a major role in the regression of mitogen (lead nitrate)-induced hepatic hyperplasia. We compared the in situ end-labeling (ISEL) technique with the conventional detection of apoptotic bodies in this process. In hematoxylin and eosin (H&E) sections, apoptosis is usually recognizable by the presence of apoptotic bodies (apoptosis phase 2). Although the early phase of apoptosis (apoptosis phase 1) can be detected as a prekaryorrhectic appearance in H&E sections, it is difficult to detect and is easily overlooked. On the other hand, ISEL presents intense staining mainly in phase 1 and weak or negative staining in phase 2. Thus, simultaneous investigation by these two methods in two serial sections is the most reliable way to calculate the incidence of apoptosis and gives us precise information on the stages of apoptosis in situ. Since the colorized signals of ISEL are much easier to detect than apoptotic bodies in H&E sections, ISEL is particularly useful for liver tissues, where the incidence of apoptosis is low.

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Mar 1, 1988·The Journal of Pathology·P A HallA G Stansfeld
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R C DukeJ J Cohen
Jan 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J H WijsmanJ H van Dierendonck

❮ Previous
Next ❯

Citations

Apr 5, 2005·Journal of Toxicology and Environmental Health. Part a·Tapan K MondalDavid A Lawrence
Oct 7, 2009·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·K Narayana, Maie Al-Bader
Jan 12, 2011·Expert Opinion on Drug Safety·Victoria LinaresJosé L Domingo
Nov 7, 2013·Toxicology and Industrial Health·Izabela GutowskaEwa Stachowska
Jan 19, 2000·Pediatric Pulmonology·L BenturE Rosenthal
Dec 22, 1999·Allergy·M A GonzaloL Fernández
Apr 4, 2001·Alimentary Pharmacology & Therapeutics·P BlomqvistA Ekbom

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis