Identification of aquatic Burkholderia (Pseudomonas) cepacia by hybridization with species-specific rRNA gene probes.

Applied and Environmental Microbiology
L G LeffL J Shimkets

Abstract

Burkholderia (Pseudomonas) cepacia is a common environmental bacterium which can be pathogenic for plants and humans. In this study, four strategies were used to identify aquatic isolates: API test strips, hybridization with species-specific DNA probes for the 16S and 23S rRNA genes, fatty acid methyl ester (FAME) profiles, and growth on selective medium (TB-T agar [C. Hagedorn, W. D. Gould, T. R. Bardinelli, and D. R. Gustarson, Appl. Environ. Microbiol. 53:2265-2268, 1987]). Only 59% of the isolates identified as B. cepacia with the API test strips were confirmed as B. cepacia by using fatty acid profiles. The 23S rRNA probe generated a few false-positive results but dramatically underestimated the number of B. cepacia isolates (i.e., 40% of the colonies that did not hybridize to the probe were B. cepacia, as determined by FAME). The 16S rRNA probe generated more false-positive results than the 23S rRNA probe but was effective in identifying the majority of the B. cepacia isolates. The selective medium was only partially successful in recovering B. cepacia. Use of the B. cepacia-specific 16S rRNA probe was the most efficient and accurate way of identifying B. cepacia.

References

Dec 1, 1989·Journal of Clinical Microbiology·G M Mukwaya, D F Welch
Jan 1, 1988·Molecular & General Genetics : MGG·L J Shimkets, S J Asher
Sep 1, 1987·Applied and Environmental Microbiology·C HagedornD R Gustavson
Oct 1, 1993·Applied and Environmental Microbiology·E B Braun-HowlandS A Nierzwicki-Bauer
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J V McArthurM H Smith

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Citations

Dec 22, 2006·PloS One·Eshwar MahenthiralingamChris G Dowson
Apr 7, 2006·Microbial Ecology·Michael J Lemke, Laura G Leff
Jul 8, 2005·Applied and Environmental Microbiology·George W PayneEshwar Mahenthiralingam
May 1, 1995·Applied and Environmental Microbiology·M G WiseJ V McArthur
Sep 28, 2001·Journal of Clinical Microbiology·T CoenyeJ J LiPuma

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