Identification of AstG1, A LAL family regulator that positively controls ansatrienins production in Streptomyces sp. XZQH13

Current Microbiology
Chao XieHao-Xin Wang

Abstract

Ansamycins is a group of type I polyketides characterized by the unique starter unit 3-amino-5-hydroxybenzoic acid. This family of secondary metabolites shows diverse biological activities, well-known members of which include rifamycin, geldanamycin, and maytansine. Previously, we isolated an AHBA synthase gene-positive strain Streptomyces sp. XZQH13 containing a "silent" ansamycin biosynthetic gene cluster ast. The constitutive expression of the Large-ATP-binding regulators of the LuxR family regulator gene astG1 located within the cluster triggered the expression of the biosynthetic genes. Reverse transcription-PCR experiments showed that the expression of the key biosynthetic genes, astB4, astD1, and astF1, was induced in the astG1 overexpression mutant compared to the wild type. This led to the isolation of two known ansatrienins, hydroxymycotrienin A (1) and thiazinotrienomycin G (2), which were identified by analysis of the mass spectral and NMR spectral data, from the mutant. These observations suggest that astG1 is probably a pathway-specific positive regulator for the biosynthesis of ansatrienin.

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Citations

Nov 11, 2016·Applied Microbiology and Biotechnology·Zhenhong ZhuYongquan Li
Jan 25, 2019·Drug Discoveries & Therapeutics·Zhongyue LiYuemao Shen
Dec 2, 2017·Chembiochem : a European Journal of Chemical Biology·Jianxiong WangYuemao Shen
Mar 7, 2020·Frontiers in Bioengineering and Biotechnology·Jia-Wei LiYun-Peng Bai
Jul 22, 2019·Applied Microbiology and Biotechnology·Yue-Ping LiYong-Quan Li
Dec 17, 2019·Chemical Reviews·Aleksandra NivinaChaitan Khosla
Apr 14, 2021·Annual Review of Biochemistry·Brett C CovingtonMohammad R Seyedsayamdost
Sep 17, 2019·Organic Letters·Feifei WeiYuemao Shen

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