Identification of AtENT3 as the main transporter for uridine uptake in Arabidopsis roots

Cell Research
Kun Ling ChenDaowen Wang

Abstract

Previous studies have shown that Arabidopsis equilibrative nucleoside transporters (AtENTs) possess transport activities when produced in yeast cells and are differentially expressed in Arabidopsis organs. Herein, we report further analysis on the nucleoside transport activities and transcriptional patterns of AtENT members. The recombinant proteins of AtENTs 3, 6, and 7, but not those of AtENTs 1, 2, 4, and 8, were found to transport thymidine with high affinity. Contrary to previous suggestion that AtENT1 may not transport uridine, this work showed that recombinant AtENT1 was a pH-dependent and high-affinity transporter of uridine. When grown on MS plates, the AtENT3 knockout plants were more tolerant to the cytotoxic uridine analog 5-fluorouridine than wild-type plants and the knockout plants of AtENT1 or AtENT6. Consistent with this observation, the AtENT3 knockout line exhibited a significantly decreased ability to take up [(3)H]uridine via the roots when compared with wild-type plants and the plants with mutated AtENT1 or AtENT6. This indicates that AtENT3, but not AtENTs 1 and 6, is the main transporter for uridine uptake in Arabidopsis roots. The transcription of AtENTs 1, 3, 4, 6, 7, and 8 was regulated in a complex ma...Continue Reading

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Citations

Jan 6, 2009·FEBS Letters·Tyler A MansfieldGeorge S Mourad
Jan 18, 2011·The Plant Journal : for Cell and Molecular Biology·Benjamin JungTorsten Möhlmann
Jun 17, 2015·Biochimica Et Biophysica Acta·Christopher GirkeM Joanne Lemieux
Sep 25, 2014·Frontiers in Plant Science·Christopher GirkeTorsten Möhlmann
Nov 21, 2018·Frontiers in Plant Science·Vanessa Jane MelinoSigrid Heuer
Sep 10, 2020·Journal of Experimental Botany·Zakayo KazibweDiane C Bassham

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