Identification of axolotl BH3-only proteins and expression in axolotl organs and apoptotic limb regeneration tissue

Biology Open
Vesna BucanSarah Strauß

Abstract

Like other urodela amphibians, axolotls are able to regenerate lost appendages, even as adults, rendering them unique among higher vertebrates. In reaction to the severe trauma of a lost limb, apoptosis seems to be primarily implicated in the removal of injured cells and tissue homeostasis. Little, however, is known about apoptotic pathways and control mechanisms. Therefore, here we provide additional information regarding the mechanisms of tissue degradation. Expression patterns of Bcl-2 family members were analyzed using reverse transcriptase-PCR, western blotting and immunofluorescence. In our study, we identified ten putative axolotl orthologs of the Bcl-2 family. We demonstrated that BH3-only proteins are differentially expressed in some axolotl organs, while they are expressed broadly in tail composite tissue and limb regeneration blastema. The importance of Bcl-2 family members is also indicated by detecting the expression of proapoptotic protein Bak in spatial congruence to apoptosis in the early stages of limb regeneration, while Bcl-2 expression was slightly modified. In conclusion, we demonstrate that Bcl-2 family members are conserved in the axolotl and might be involved in the tissue degradation processes that occu...Continue Reading

References

Sep 1, 1976·Journal of Morphology·P W TankT G Connelly
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Oct 24, 1998·The International Journal of Biochemistry & Cell Biology·D G Greenhalgh
Nov 21, 1998·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·E M Tanaka, J P Brockes
Nov 24, 1999·Brain Research. Developmental Brain Research·Y KanekoY Hanyu
May 16, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·A L MescherJ J Brokaw
Apr 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·E Salas-VidalL Covarrubias
May 3, 2003·Biochemical and Biophysical Research Communications·Yueh-Chun HsiehChang-Jen Huang
May 6, 2003·Biochemical and Biophysical Research Communications·Cristina Clavería, Miguel Torres
May 16, 2003·Current Biology : CB·Yutaka Imokawa, Jeremy P Brockes
Jun 21, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D Du PasquierA Mazabraud
Aug 5, 2006·Cell Death and Differentiation·E KratzA Ashkenazi
Jul 1, 2008·Journal of Molecular Biology·Catherine L DayMark G Hinds
Jul 31, 2008·Journal of Thrombosis and Haemostasis : JTH·J J LopezJ A Rosado
Jul 31, 2009·Oncogene·E Lomonosova, G Chinnadurai
Dec 2, 2009·BMC Biology·Nandini RaoDavid L Stocum
Dec 25, 2009·Apoptosis : an International Journal on Programmed Cell Death·Peter M Eimon, Avi Ashkenazi
Apr 28, 2010·Mechanisms of Development·James W GodwinJeremy P Brockes
Mar 2, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jessica L WhitedClifford J Tabin
May 10, 2011·Nature·Garabet YeretssianMaya Saleh

❮ Previous
Next ❯

Citations

Mar 10, 2018·Journal of Neural Engineering·Zachariah J SperryTim M Bruns
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Dylan J GuerinKelly Ai-Sun Tseng

❮ Previous
Next ❯

Methods Mentioned

BETA
amputation
PCR
electrophoresis

Software Mentioned

WebLOGOs
WebLOGO
ClustalW2
qbasePlus
Treeview
coffee
BLAST

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis