Identification of B cell and T cell epitopes using synthetic peptide combinatorial libraries

Current Protocols in Immunology
C PinillaR A Houghten

Abstract

This unit presents a combinatorial library method that consists of the synthesis and screening of mixture-based synthetic combinatorial libraries (SCL) of peptide molecules. The protocols employ peptide SCL to identify peptides recognized by mAbs and T cells. The first protocol uses a positional scanning SCL (PS-SCL) made up of hexapeptides to identify antigenic determinants recognized by mAbs. The 120 mixtures in the hexapeptide PS-SCL are tested for their inhibitory activity in a competitive ELISA. The second protocol uses a decapeptide PS-SCL to identify T cell peptide ligands. The 200 mixtures of the decapeptide PS-SCL are tested for their ability to induce proliferation. Support protocols cover optimization of the assay conditions for each mAb or T cell, to achieve the best level of sensitivity and reproducibility, and preparation of a hexapeptide PS-SCL, along with deconvolution approaches.

References

Jun 1, 1986·International Journal of Peptide and Protein Research·R A HoughtenC J Kirby
May 14, 1999·Current Opinion in Immunology·C PinillaR A Houghten
Oct 3, 1999·Journal of Medicinal Chemistry·R A HoughtenJ M Ostresh
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B HemmerR Martin

❮ Previous
Next ❯

Citations

Sep 21, 2010·Journal of Molecular Modeling·Austin B YongyeKarina Martínez-Mayorga

❮ Previous
Next ❯

Related Concepts

Related Feeds