PMID: 2502530May 1, 1989Paper

Identification of Bacillus anthracis by polyclonal antibodies against extracted vegetative cell antigens

The Journal of Applied Bacteriology
A P Phillips, John W Ezzell

Abstract

The extractable protein antigens EA1 and EA2 of Bacillus anthracis were prepared from electrophoresis transblots of SDS extracts of vegetative bacteria of the Sterne strain. Hyperimmune guinea-pig antiserum against EA2 failed to react with B. anthracis cells in immunofluorescence (IF) tests. Guinea-pig antiserum against EA1 (anti-EA1) reacted strongly in IF tests with non-encapsulated vegetative cell of 10 of 12 strains of B. anthracis and with cells of strains of B. cereus and B. thuringiensis. The unreactive B. anthracis strains were delta-Vollum-1B-1 and Texas. Encapsulated cells of B. anthracis stained poorly except for small bright regions. Absorption of anti-EA1 with cells of B. cereus NCTC 8035 and NCTC 9946 removed activity towards all B. cereus strains tested, but only partly reduced cross-reaction with B. thuringiensis strains. Absorption of anti-EA1 with B. thuringiensis 4041 removed activity towards this strain and B. cereus strains. Evidence is produced that B. thuringiensis cells grown on nutrient agar possess more cross-reacting antigens than cells grown in nutrient broth. The reaction of anti-EA1 with Bacillus spores immobilized in clumps on microscope slides was attributed to contaminating vegetative debris bec...Continue Reading

References

Dec 1, 1988·FEMS Microbiology Immunology·A P PhillipsR Quinn
May 1, 1986·Infection and Immunity·B E IvinsS H Leppla
Feb 1, 1988·Infection and Immunity·J W Ezzell, T G Abshire
Jun 1, 1985·Analytical Biochemistry·K A Knudsen
Jan 1, 1983·Infection and Immunity·J D Ristroph, B E Ivins
Dec 1, 1983·Applied and Environmental Microbiology·A P Phillips, K L Martin

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Citations

Nov 12, 2005·Analytical and Bioanalytical Chemistry·Katie A EdwardsAntje J Baeumner
Feb 24, 2001·Biosensors & Bioelectronics·S S IqbalJ P Chambers
Jan 1, 1992·The Journal of Applied Bacteriology·P C TurnbullJ Melling
Dec 11, 2007·Applied and Environmental Microbiology·Catherine FenselauNathan Edwards
Jul 10, 2012·Applied and Environmental Microbiology·Nathalie MorelChristophe Créminon
Oct 25, 2002·Emerging Infectious Diseases·Barun K DeTanja Popovic
Apr 20, 2010·Journal of Microbiological Methods·Shilpakala Sainath RaoChintamani D Atreya
Jan 28, 2015·The Analyst·Jahir OrozcoJoseph Wang
Oct 3, 1999·Applied and Environmental Microbiology·Y HathoutC Fenselau

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