Identification of Bacillus anthracis proteins associated with germination and early outgrowth by proteomic profiling of anthrax spores

Proteomics
Chun-Ming HuangDe-Chu C Tang

Abstract

The use of anthrax spores as a bioweapon has spurred efforts aimed at identifying key proteins expressed in Bacillus anthracis. Because spore germination and outgrowth occur prior to and are required for disease manifestations, blocking germination and early outgrowth with novel vaccines or inhibitors targeting critical B. anthracis germination and outgrowth-associated factors is a promising strategy in mitigating bioterror. By screening 587 paired protein spots that were isolated from dormant and germinating anthrax spores, respectively, we identified 10 spore proteins with statistically significant germination-associated increases and decreases. It is likely that proteins whose levels change during germination may play key roles in the germination and outgrowth processes, and they should be listed as priority targets for development of prophylactic and therapeutic agents against anthrax. The 31 new proteins identified in this study also complement an emerging proteomic database of B. anthracis.

Citations

May 1, 2012·Analytical and Bioanalytical Chemistry·M Cestelli GuidiR Amendola
May 6, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kinfai AuRobert M Esnouf
Feb 27, 2007·Journal of Bacteriology·Bart J F KeijserStanley Brul
Aug 22, 2009·BMC Structural Biology·Rafael M CouñagoKurt L Krause
Jul 31, 2007·Future Microbiology·Karla D Passalacqua, Nicholas H Bergman
Oct 15, 2009·Future Microbiology·Theodor Chitlaru, Avigdor Shafferman
Nov 12, 2013·Journal of Bacteriology·Kathryn J PflughoeftTheresa M Koehler
May 17, 2005·Expert Review of Proteomics·Celia G ZhangSandra L McCutchen-Maloney
Sep 23, 2009·Expert Opinion on Biological Therapy·Dennis M KlinmanKoji Tomaru
Feb 11, 2015·Molecular Cell·Lior SinaiSigal Ben-Yehuda
Aug 22, 2015·Journal of Microbiological Methods·Brooke L Deatherage KaiserJanine R Hutchison
Nov 19, 2005·Proteomics·Junjun WangLiuyu Huang
Aug 23, 2008·Journal of Mass Spectrometry : JMS·Plamen A Demirev, Catherine Fenselau
Aug 12, 2014·FEMS Microbiology Letters·Wishwas AbhyankarChris G de Koster
Jan 1, 2014·Molecular & Cellular Proteomics : MCP·Jérôme ChenauFrançois Becher
Oct 12, 2018·Environmental Science and Pollution Research International·Charlotte MappaBéatrice Alpha-Bazin

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