Identification of bacterial populations in dairy wastewaters by use of 16S rRNA gene sequences and other genetic markers

Applied and Environmental Microbiology
Jeffery A McGarveyL H Stanker

Abstract

Hydraulic flush waste removal systems coupled to solid/liquid separators and circulated treatment lagoons are commonly utilized to manage the large amounts of animal waste produced on high-intensity dairy farms. Although these systems are common, little is known about the microbial populations that inhabit them or how they change as they traverse the system. Using culture-based and non-culture-based methods, we characterized the microbial community structure of manure, water from the separator pit, and water from the circulated treatment lagoon from a large dairy in the San Joaquin Valley of California. Our results show that both total bacterial numbers and bacterial diversity are highest in manure, followed by the separator pit water and the lagoon water. The most prevalent phylum in all locations was the Firmicutes (low-G+C, gram-positive bacteria). The most commonly occurring operational taxonomic unit (OTU) had a 16S rRNA gene (rDNA) sequence 96 to 99% similar to that of Clostridium lituseburense and represented approximately 6% of the manure derived sequences, 14% of the separator pit-derived sequences and 20% of the lagoon-derived sequences. Also highly prevalent was an OTU with a 16S rDNA sequence 97 to 100% similar to t...Continue Reading

References

Apr 1, 1992·American Journal of Physical Medicine & Rehabilitation·S A YablonK Fruhmann
Jan 1, 1988·Dermatologica·R L GillumA J Strano
Aug 1, 1995·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·L M O'Reilly, C J Daborn
Aug 1, 1997·Molecular and Cellular Probes·R F WangC E Cerniglia
Nov 15, 1997·Journal of Dairy Science·A N Pell
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A VentosaA Oren
Oct 6, 1998·Applied and Environmental Microbiology·M F Polz, C M Cavanaugh
Oct 3, 1999·Applied and Environmental Microbiology·A BubertM Wagner
Mar 4, 2000·Applied and Environmental Microbiology·J V Gagliardi, J S Karns
May 2, 2000·Applied and Environmental Microbiology·I V WesleyI Siddique
May 2, 2000·Journal of Clinical Microbiology·M A PassR M Batt
Apr 20, 2001·Preventive Veterinary Medicine·L D WarnickM J Hawkins
Jun 21, 2001·International Journal of Systematic and Evolutionary Microbiology·S Ntougias, N J Russell
Dec 26, 2001·Bioinformatics·S KumarM Nei
Jan 26, 2002·Journal of Toxicology and Environmental Health. Part a·Len RitterBeth Linton
Feb 2, 2002·Applied and Environmental Microbiology·Thomas D LeserKristian Møller
Mar 6, 2002·Systematic and Applied Microbiology·R PukallE Stackebrandt
Aug 3, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Michael YaegerLorraine Hoffman
Aug 17, 2002·Journal of Food Protection·Tongrui LiuJohn J Maurer
Dec 4, 2002·Environmental Microbiology·Jennifer E BrofftLawrence J Shimkets
May 15, 2003·The Journal of Emergency Medicine·Benjamin S AbellaDavid S Howes
Jun 6, 2003·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·E J Manning
Aug 29, 2003·Annales de chirurgie·P Carron, D Tagan
Sep 6, 2003·Applied and Environmental Microbiology·A Mark IbekweStephen R Lyon
Oct 9, 2003·Applied and Environmental Microbiology·Anushree MalikKazuo Kakii

❮ Previous
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Citations

Jul 5, 2012·Applied Microbiology and Biotechnology·Feng Guo, Tong Zhang
Nov 10, 2011·The Journal of Microbiology·Jesús Andrei Rosales-CastilloGerardo Vázquez-Marrufo
Nov 1, 1992·Mechanisms of Development·B Christen, M Bienz
May 18, 2010·Applied and Environmental Microbiology·Lisa M DursoMichael L Clawson
Sep 2, 2008·Applied and Environmental Microbiology·Marie-Claude JulienPascal Drouin
Nov 7, 2006·Applied and Environmental Microbiology·Jeffery A McGarveyFrank Mitloehner
Jan 30, 2007·Applied and Environmental Microbiology·Céline DelbèsMarie-Christine Montel
Jul 21, 2009·Applied and Environmental Microbiology·Elizabeth RettedalVolker S Brözel
Nov 15, 2011·Applied and Environmental Microbiology·Isabelle Verdier-MetzMarie-Christine Montel
Aug 14, 2012·The Science of the Total Environment·Moez BoualiAbdelghani Sghir
Jan 15, 2013·Journal of Applied Microbiology·H MiyamotoH Kodama
Jan 1, 2011·Journal of Environmental Sciences (China)·Abd El-latif HeshamMin Yang
Jul 28, 2016·Environmental Technology·Dhan Prasad GautamAchintya N Bezbaruah
Aug 30, 2016·International Journal of Environmental Research and Public Health·Christy E Manyi-LohAnthony I Okoh
Jul 18, 2015·International Journal of Environmental Research and Public Health·Subbarao V Ravva, Anna Korn
Nov 30, 2014·Microbial Ecology·Marcio Luis Busi Da SilvaCarlos Wolfgang Nossa
May 28, 2019·Journal of Environmental Sciences (China)·Niloy Chandra SarkerShafiqur Rahman

❮ Previous
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