Identification of Bartonella (Rochalimaea) species among fastidious gram-negative bacteria on the basis of the partial sequence of the citrate-synthase gene.

Journal of Clinical Microbiology
C JobletD Raoult

Abstract

The bacterial genus Bartonella (Rochalimaea) includes emerging human pathogens with five recognized species. These are fastidious gram-negative bacteria, exhibiting few phenotypic characteristics and whose identification relies upon serotyping, cellular fatty acid analysis, and molecular typing. Most of the isolates have been recovered from the blood of patients, and three of the four pathogenic Bartonella species are associated with infectious endocarditis. We performed PCR-restriction fragment length polymorphism (RFLP) analysis of the blood culture bottle supernatant for the routine identification of Bartonella species among fastidious gram-negative bacteria. The amplification of the citrate-synthase gene with primers previously reported (R. L. Regnery, C. L. Spruill, and B. D. Plikaytis, J. Bacteriol. 173:1576-1589, 1991) yielded a 379-bp product from Bartonella species and a 382-bp product for Capnocytophaga ochracea but no product from any of the other 15 genotypically or phenotypically related species tested. We determined the sequences of the citrate-synthase gene-amplified products for Bartonella species and C. ochracea in order to predict the optimal restriction enzyme to be used in RFLP analysis. TaqI and AciI allowe...Continue Reading

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Citations

May 8, 2001·American Journal of Clinical Pathology·H LepidiD Raoult
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·Renaud MaillardMuriel Vayssier-Taussat
Jul 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M MaurinD Raoult
Jan 5, 2000·Clinical and Diagnostic Laboratory Immunology·Z Liang, D Raoult
Oct 29, 2005·Journal of Molecular Microbiology and Biotechnology·Ricardo G Maggi, Edward B Breitschwerdt
Oct 11, 2013·Ocular Immunology and Inflammation·Ana Luiza Biancardi, Andre Luiz Land Curi
Oct 29, 1998·AIDS·S GasquetD Raoult
Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Florence Fenollar, Didier Raoult
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Dec 1, 2017·Clinical Microbiology Reviews·Cláudia Gomes, Joaquim Ruiz
Jan 7, 2005·Journal of Clinical Microbiology·Clarisse RoveryDidier Raoult
Mar 8, 2005·Journal of Clinical Microbiology·Ricardo G Maggi, Edward B Breitschwerdt
Apr 1, 1997·Clinical Microbiology Reviews·B E Anderson, M A Neuman
Jun 16, 2005·Journal of Clinical Microbiology·Ricardo G MaggiEdward B Breitschwerdt
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