Identification of basal promoter and enhancer elements in an untranslated region of the TT virus genome

Journal of Virology
Tetsuro SuzukiTatsuo Miyamura

Abstract

The regulation of TT virus (TTV) gene expression was characterized. Transient-transfection assays using reporter constructs revealed that a 113-nucleotide (nt) sequence within the untranslated region, proximal to the transcription initiation site and containing a TATA box motif, has a basal promoter activity. This sequence is well conserved among different TTV genotypes. Upstream stimulating factor bound to a consensus binding motif within this region and positively regulates TTV transcription. Furthermore, a 488-nt region upstream of the basal promoter exhibited enhancer activity, presumably in a cell type-specific manner. This study illustrates some of the mechanisms involved in the transcriptional regulation of TTV.

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Citations

Nov 18, 2000·Cellular Signalling·W J van Blitterswijk, B Houssa
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Nov 22, 2019·Veterinary World·Vinutha SubramanyamHabibur Rahaman
May 15, 2020·Virus Research·Brett WebbSheela Ramamoorthy

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