Identification of bases required for P2 integrase core binding and recombination

Virology
Lina SylwanE Haggård-Ljungquist

Abstract

Temperate coliphage P2 integrates its genome into the host chromosome upon lysogenization via a site-specific recombination event mediated by an integrase belonging to the complex family of tyrosine recombinases. The host integration site attB (BOB') is localized in the end of the cyaR gene and shares 27 nucleotides with the core of attP (COC'). In the present study we determine the minimal attB site using an in vivo recombination assay. Ten nt on the left side (B) are found to be nonessential for recombination. We show that the integrase has higher affinity for the right side (B') compared to B and that artificial B'OB' and an attP site with a matching core (C'OC') are efficient substrates for recombination in vitro. We have analyzed single nucleotides in attB and find that sequence homology within a non-centrally located quadruplet in the hypothetical overlap region is essential for efficient recombination in vivo.

References

Sep 1, 1965·Journal of General Microbiology·I Sasaki, G Bertani
Jul 11, 2001·Genes & Development·K M WassarmanS Gottesman
Jan 22, 2005·Virology·Clara FrumerieElisabeth Haggård-Ljungquist
Dec 22, 2005·Current Opinion in Structural Biology·Marta Radman-LivajaHideki Aihara
Feb 21, 2008·Journal of Applied Microbiology·C FrumerieE Haggård-Ljungquist
Nov 4, 2008·Journal of Bacteriology·Nicholas De Lay, Susan Gottesman
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Lara RajeevJeffrey F Gardner
Dec 3, 2009·Nucleic Acids Research·Jennifer LapriseJeffrey F Gardner

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Citations

May 27, 2014·Journal of Molecular Biology·Paul C M FoggMargaret C M Smith
Apr 27, 2016·Journal of Molecular Biology·Bryce L Lunt, Graham F Hatfull
Nov 10, 2020·Frontiers in Microbiology·Mohammed Radhi MohaisenHeather Elizabeth Allison

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