Identification of basophils by a mAb directed against pro-major basic protein 1

The Journal of Allergy and Clinical Immunology
Douglas A PlagerKristin M Leiferman

Abstract

Basophils possess characteristics of both mast cells and eosinophils, and all 3 cell types often are found together, particularly during allergic reactions. A mAb (J175-7D4) generated against the recombinant pro-form of human eosinophil granule major basic protein 1 (rproMBP1) appeared to stain only basophils in tissue specimens. We investigated J175-7D4 to characterize its specificity for basophils. Fluid-phase immunoprecipitation, Western blotting, and immunocytochemistry and immunohistochemistry were used to establish the specificity of J175-7D4. First, J175-7D4 binds to various glycosylated and proteolytically processed forms of rproMBP1, but not to major basic protein. Second, cells transfected with the rproMBP1 gene and human placental tissue (known to express the pro-form of major basic protein 1 [proMBP1]) stain specifically with J175-7D4. In contrast, although mature eosinophils contain substantial major basic protein, they lack proMBP1 and do not stain. Neutrophils, lymphocytes, monocytes, and skin mast cells also are not stained. However, blood basophils are stained by J175-7D4, anti-IgE, Wright-Giemsa (metachromatically), and a previously characterized basophil-specific mAb, 2D7. Finally, formaldehyde-fixed, paraffi...Continue Reading

References

Mar 1, 1976·The Journal of Clinical Investigation·G J GleichJ E Maldonado
Aug 1, 1990·International Journal for Parasitology·E B Steeves, J R Allen
Feb 8, 1989·Journal of Immunological Methods·K D KrenikG J Gleich
May 1, 1989·The Journal of Clinical Investigation·E N CharlesworthL M Lichtenstein
Jan 1, 1989·International Archives of Allergy and Applied Immunology·T IshizakaK Ishizaka
Mar 1, 1974·Journal of Immunological Methods·N W Johnston, J Bienenstock
Feb 1, 1972·The Journal of Experimental Medicine·H F Dvorak, M C Mihm
Sep 1, 1983·The Journal of Experimental Medicine·S J AckermanG J Gleich
Jul 1, 1984·The Journal of Experimental Medicine·D E MaddoxG J Gleich
Feb 10, 1994·Journal of Immunological Methods·M IdeG J Gleich
May 13, 1999·The Journal of Biological Chemistry·D A PlagerG J Gleich
Nov 7, 1999·The Journal of Allergy and Clinical Immunology·P LozanoK B Weiss
Dec 5, 2000·Current Opinion in Immunology·J WedemeyerS J Galli
Apr 9, 2001·Immunological Reviews·B S Bochner, R P Schleimer
Oct 6, 2001·American Journal of Respiratory and Critical Care Medicine·C L KepleyM F Lipscomb
Mar 15, 2002·Current Allergy and Asthma Reports·Donald MacGlashanJohn T Schroeder

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Citations

Jul 11, 2013·Forensic Science, Medicine, and Pathology·Erik Edston
Dec 21, 2010·Annual Review of Immunology·Hajime KarasuyamaTakeshi Wada
Feb 24, 2016·The World Allergy Organization Journal·Dean D MetcalfeMassimo Triggiani
Oct 1, 2013·The Journal of Allergy and Clinical Immunology·Mark C SiracusaDavid Artis
Aug 21, 2009·Kidney International·Matthias Mack, Alexander R Rosenkranz
Nov 1, 2011·Current Opinion in Immunology·David Voehringer
Mar 5, 2010·The Journal of Allergy and Clinical Immunology·F Estelle R Simons
Sep 29, 2009·Trends in Parasitology·David Voehringer
Feb 7, 2014·The Journal of Investigative Dermatology·Francesco BorrielloGianni Marone
Nov 26, 2009·Dermatologic Therapy·Nicoletta CassanoGino Antonio Vena
Jun 21, 2011·Immunological Reviews·John T Schroeder
Jun 15, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J K Lee, P Vadas
Jun 30, 2011·The American Journal of Pathology·Tsukasa UgajinHiroo Yokozeki
Mar 19, 2014·The Journal of Allergy and Clinical Immunology·Mahboobeh MahdaviniaRobert P Schleimer
Mar 25, 2015·Molecular Immunology·Tsukasa UgajinToshio Hirano
Jan 21, 2009·Allergology International : Official Journal of the Japanese Society of Allergology·Kaori MukaiHajime Karasuyama
Sep 14, 2016·Biomédica : revista del Instituto Nacional de Salud·Daniel AmayaJorge Sánchez
Jan 14, 2021·Nature Communications·Sho HiroyasuDavid J Granville

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