Identification of benzo[a]pyrene-induced cell cycle-associated alterations in MCF-7 cells using infrared spectroscopy with computational analysis

Toxicology
Weiyi PangFrancis L Martin

Abstract

Chemical contaminants, such as benzo[a]pyrene (B[a]P), may modulate transcriptional responses in cells via the activation of aryl hydrocarbon receptor (AhR) or through responses to DNA damage following adduct formation. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy can be employed in a non-destructive fashion to interrogate the biochemical signature of cells via generation of infrared (IR) spectra. By applying to generated spectral datasets subsequent computational approaches such as principal component analysis plus linear discriminant analysis (PCA-LDA), derived data reduction is achieved to facilitate the visualization of wavenumber-related alterations in target cells. Discriminating spectral variables might be associated with lipid or glycogen content, conformational protein changes and phosphorylation, and structural alterations in DNA/RNA. Using this approach, we investigated the dose-related effects of B[a]P in MCF-7 cells concentrated in S- or G₀/G₁-phase. Our findings identified that in PCA-LDA scores plots a clear segregation of IR spectra was evident, with the major spectral alterations associated with DNA/RNA, secondary protein structure and lipid. Dose-related effects were observed ...Continue Reading

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Citations

Mar 26, 2015·Expert Review of Molecular Diagnostics·Georgios TheophilouFrancis L Martin
Dec 22, 2012·Environmental Pollution·Blessing E Obinaju, Francis L Martin
Jul 17, 2014·The Analyst·Graeme ClemensMatthew J Baker
Nov 28, 2018·Environmental Science and Pollution Research International·Pingjing WenQijun Wu

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