Identification of beta-Lactamases and beta-Lactam-Related Proteins in Human Pathogenic Bacteria using a Computational Search Approach

Current Microbiology
Aniel Jessica Leticia Brambila-TapiaMario Salazar-Páramo

Abstract

A systematic analysis of beta-lactamases based on comparative proteomics has not been performed thus far. In this report, we searched for the presence of beta-lactam-related proteins in 591 bacterial proteomes belonging to 52 species that are pathogenic to humans. The amino acid sequences for 19 different types of beta-lactamases (ACT, CARB, CifA, CMY, CTX, FOX, GES, GOB, IMP, IND, KPC, LEN, OKP, OXA, OXY, SHV, TEM, NDM, and VIM) were obtained from the ARG-ANNOT database and were used to construct 19 HMM profiles, which were used to identify potential beta-lactamases in the completely sequenced bacterial proteomes. A total of 2877 matches that included the word "beta-lactamase" and/or "penicillin" in the functional annotation and/or in any of its regions were obtained. These enzymes were mainly described as "penicillin-binding proteins," "beta-lactamases," and "metallo-beta-lactamases" and were observed in 47 of the 52 species studied. In addition, proteins classified as "beta-lactamases" were observed in 39 of the species included. A positive correlation between the number of beta-lactam-related proteins per species and the proteome size was observed (R 0.78, P < 0.00001). This correlation partially explains the high presence ...Continue Reading

References

Jul 1, 1982·Journal of Bacteriology·A G BarbourH D Caldwell
Sep 1, 2006·Current Opinion in Microbiology·Rafael Cantón, Teresa M Coque
Jul 17, 2007·Clinical Microbiology Reviews·Anne Marie Queenan, Karen Bush
Dec 25, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M Gniadkowski
Feb 21, 2009·Bioinformatics·Alexandra MouraAntónio Correia
Apr 23, 2009·BMC Bioinformatics·Michael J JossYan Boucher
Sep 9, 2009·Biochemistry·Pattraranee LimphongMichael W Crowder
Feb 5, 2011·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Magdalena Chroma, Milan Kolar
Dec 6, 2011·Database : the Journal of Biological Databases and Curation·Guy TsafnatSally R Partridge
Aug 17, 2012·Current Pharmaceutical Design·Darren Trott
Jan 26, 2013·Annals of the New York Academy of Sciences·Karen Bush
May 8, 2013·Antimicrobial Agents and Chemotherapy·Andrew G McArthurGerard D Wright
Oct 23, 2013·Antimicrobial Agents and Chemotherapy·Sushim Kumar GuptaJean-Marc Rolain
Jan 9, 2014·The Journal of Antimicrobial Chemotherapy·Sam AbrahamDarren J Trott
Apr 16, 2014·BMC Bioinformatics·Jean-Pierre FlandroisOana Dumitrescu

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Software Mentioned

SPSS
HMMbuild
ANNOT
HMMsearch
ARG

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