Identification of beta1,4GalT II as a target gene of p53-mediated HeLa cell apoptosis

Journal of Biochemistry
Jin ZhouJianxin Gu

Abstract

beta1,4-galactosyltransferase II (beta1,4GalT II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Previously, we have reported that beta1,4GalT II overexpression increased cisplatin-induced HeLa cell apoptosis. However, the mechanisms of its expression regulation have been rarely investigated. Here, we cloned the 1.8-kb 5'-flanking region of the beta1,4GalT II gene and analysed its promoter activity. The transcriptional activity and mRNA expression level of beta1,4GalT II were dramatically induced by p53 transcription factor in HeLa cells. In response to DNA damage agent adriamycin, the mRNA expression and promoter activity of beta1,4GalT II were significantly up-regulated and the binding of p53 to beta1,4GalT II promoter was obviously increased. Furthermore, decreasing the expression of beta1,4GalT II using RNA interference inhibited p53-mediated HeLa cell apoptosis induced by adriamycin. Collectively, these results suggested that beta1,4GalT II might serve as a target gene of p53 transcription factor during adriamycin-induced HeLa cell apoptosis, which elucidated a new mechanism of p53-mediated cell apoptosis.

Citations

Jun 17, 2010·Journal of Biochemistry·Fengbiao ZhouYuanyan Wei
Feb 24, 2017·Biological & Pharmaceutical Bulletin·Atena SugiyamaTakeshi Sato
Feb 25, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Haijian ZhangJiejie Xu
Mar 7, 2015·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Kiyoshi Furukawa
Oct 20, 2019·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Aziz Ur Rehman AzizBo Liu

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Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis