Identification of BFN1, a bifunctional nuclease induced during leaf and stem senescence in Arabidopsis

Plant Physiology
M A Pérez-AmadorPamela J Green

Abstract

Nuclease I enzymes are responsible for the degradation of RNA and single-stranded DNA during several plant growth and developmental processes, including senescence. However, in the case of senescence the corresponding genes have not been reported. We describe the identification and characterization of BFN1 of Arabidopsis, and demonstrate that it is a senescence-associated nuclease I gene. BFN1 nuclease shows high similarity to the sequence of a barley nuclease induced during germination and a zinnia (Zinnia elegans) nuclease induced during xylogenesis. In transgenic plants overexpressing the BFN1 cDNA, a nuclease activity of about 38 kD was detected on both RNase and DNase activity gels. Levels of BFN1 mRNA were extremely low or undetectable in roots, leaves, and stems. In contrast, relatively high BFN1 mRNA levels were detected in flowers and during leaf and stem senescence. BFN1 nuclease activity was also induced during leaf and stem senescence. The strong response of the BFN1 gene to senescence indicated that it would be an excellent tool with which to study the mechanisms of senescence induction, as well as the role of the BFN1 enzyme in senescence using reverse genetic approaches in Arabidopsis.

References

Dec 1, 1992·European Journal of Biochemistry·S Gite, V Shankar
Oct 18, 1991·Biochemistry·J A StricklandP W Doetsch
Sep 15, 1991·European Journal of Biochemistry·K MaekawaF Sakiyama
Jul 1, 1991·Journal of Biochemistry·A IwamatsuF Sakiyama
Oct 15, 1987·European Journal of Biochemistry·P H Brown, T H Ho
Jun 1, 1987·Archives of Biochemistry and Biophysics·I Naseem, S M Hadi
Jan 1, 1972·Annual Review of Biochemistry·R D Marshall
Jan 1, 1980·Methods in Enzymology·M Laskowski
Jun 1, 1982·Archives of Biochemistry and Biophysics·A E OlesonT A Wiesner
Jan 1, 1995·Critical Reviews in Microbiology·S U Gite, V Shankar
Jun 1, 1995·Bioscience, Biotechnology, and Biochemistry·H KobayashiM Irie
Nov 1, 1994·The Plant Journal : for Cell and Molecular Biology·P A BariolaP J Green
Feb 25, 1993·Nucleic Acids Research·C el AdlouniA Przykorska
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C B TaylorP J Green
Sep 1, 1996·The Plant Journal : for Cell and Molecular Biology·A van Hoof, P J Green
Oct 1, 1996·Plant Molecular Biology·M Bar-PeledN V Raikhel
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 4, 1998·Plant Molecular Biology·A LersP J Green
Mar 6, 1999·Current Opinion in Plant Biology·A B Bleecker
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Blank, T A McKeon

❮ Previous
Next ❯

Citations

Dec 18, 2004·Plant Molecular Biology·Zhong ChenDaniel R Gallie
Oct 15, 2003·Trends in Plant Science·Preeti Dahiya
Apr 18, 2012·Journal of Agricultural and Food Chemistry·Chia-Yun KoJei-Fu Shaw
Sep 6, 2000·The Plant Journal : for Cell and Molecular Biology·K RihaD E Shippen
Feb 16, 2002·The Plant Journal : for Cell and Molecular Biology·Nicole D LeBrasseurPamela J Green
Dec 7, 2006·Plant Biotechnology Journal·Vicky Buchanan-WollastonDavid Pink
Jan 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Melissa S HillwigGustavo C Macintosh
Jan 4, 2012·Journal of Experimental Botany·Benjamin BollhönerHannele Tuominen
May 14, 2004·Nucleic Acids Research·Bradley J TillSteven Henikoff
Apr 27, 2013·Nucleic Acids Research·Anna GoliszJoanna Kufel
Apr 27, 2013·Plant & Cell Physiology·Krzysztof LesniewiczElzbieta Poreba
Feb 7, 2013·Acta Crystallographica. Section D, Biological Crystallography·Tomáš Koval'Jan Dohnálek
Jan 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tomáš Koval'Jan Dohnálek
Jul 21, 2012·Biochemistry. Biokhimii︠a︡·L I Fedoreyeva, B F Vanyushin
Feb 6, 2013·Biochemistry. Biokhimii︠a︡·L I Fedoreyeva, B F Vanyushin
Oct 30, 2012·BMC Plant Biology·Krzysztof LeśniewiczPrzemysław Wojtaszek
Nov 22, 2013·BMC Plant Biology·Núria Sánchez-Pons, Carlos M Vicient
Nov 2, 2013·Planta·Waseem Shahri, Inayatullah Tahir
Mar 19, 2014·Journal of Experimental Botany·Wataru Sakamoto, Tsuneaki Takami
Jul 17, 2001·Physiologia Plantarum·Amnon LersAndrei Khalchitski
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Tomáš PodzimekJiří Santrůček
Mar 23, 2004·Physiologia Plantarum·Fitzgerald L. Booker
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Karine TriquesAbdelhafid Bendahmane
Jun 15, 2012·Journal of Integrative Plant Biology·Hui-Min ChenXin-Qiang He

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.