Identification of Bifidobacterium strains by rRNA gene restriction patterns.

Applied and Environmental Microbiology
I ManginB Decaris

Abstract

Total DNA from 21 collection or industrial Bifidobacterium strains was cleaved with various restriction endonucleases. Following electrophoresis, the fragments were subjected to Southern blot hybridization with a heterologous [alpha-32P]dCTP-labeled rDNA (genes coding for rRNA) 23S gene probe. The ribosomal patterns allowed all tested strains to be differentiated and previous classifications to be confirmed. The same method was used to characterize DNA from 121 Bifidobacterium isolates collected from the intestinal flora of five human volunteers after the induction of colonic bacterial imbalance by antibiotics and absorption of a resistant exogenous Bifidobacterium strain. Hybridizations with the ribosomal probe revealed 11 different ribosomal patterns in addition to that of the exogenous strain. They permitted the Bifidobacterium populations belonging to the dominant colonic flora to be monitored over time. This experiment revealed significant and sustained alterations of the endogenous intestinal flora; indeed, some strains were eliminated, while others, probably belonging to subdominant flora, replaced them. Furthermore, even 2 months after the end of antibiotic treatment, the colonic flora remained different from that obser...Continue Reading

References

Aug 1, 1991·Applied and Environmental Microbiology·A O Baloga, S K Harlander
Apr 1, 1991·Gut·R Fuller
Sep 1, 1986·Annales De L'Institut Pasteur. Microbiology·F Grimont, P A Grimont
Nov 1, 1989·Research in Microbiology·F GrimontP A Grimont
Aug 1, 1993·The Proceedings of the Nutrition Society·J C RambaudP Pochart
Mar 1, 1952·Journal of Bacteriology·J LEDERBERG, E M LEDERBERG
Jun 1, 1989·Revue Scientifique Et Technique·R Ducluzeau, P Raibaud

❮ Previous
Next ❯

Citations

Jan 1, 1999·Journal of Applied Microbiology·N DesmasuresM Guéguen
Nov 11, 2005·The American Journal of Gastroenterology·Giovanni BarbaraRoberto Corinaldesi
Jun 3, 2008·The American Journal of Gastroenterology·Gareth C ParkesJeremy D Sanderson
Nov 14, 2015·Critical Reviews in Food Science and Nutrition·Yao Mianzhi, Nagendra P Shah
Mar 26, 1998·FEMS Microbiology Letters·K O'Riordan, G F Fitzgerald
Jan 26, 2002·The American Journal of Gastroenterology·P R MaxwellM A Mendall
Jan 20, 2006·FEMS Microbiology Ecology·Irène ManginPhilippe Pochart
Jul 12, 2003·FEMS Microbiology Letters·Koen Venema, Annet J H Maathuis
Apr 18, 2003·Medical Hypotheses·Alan C LoganDinaz Irani
Apr 20, 2001·Journal of Agricultural and Food Chemistry·D J O'Sullivan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.