Identification of "big" human placental lactogen in placenta and serum

Endocrinology
A B SchneiderL M Sherwood

Abstract

Because of increasing evidence for the heterogeneity of polypeptide hormones, studies of the molecular species of human placental lactogen (hPL) were initiated. When extracts of freshly delivered human placentas were passed over Sephadex G-100 in 0.05M ammonium carbonate, three immunoreactive peaks were detected. In addition to a peak corresponding to native hPL (Kav = 0.39) and one in the void volume, a consistent peak which eluted before hPL (Kav = 0.20) was present. The latter represented 2-25% of total hormonal activity and could be rerun without significant conversion to hPL. In 8M urea, the peak continued to behave as a large molecular weight form on both Sephadex chromatography and on polyacrylamide disc gel electrophoresis. Extraction procedures at both neutral and alkaline pH produced similar quantities of the larger material. [125I]iodo-hPL was not converted to the larger form by the conditions of extraction or analysis. These properties are consistent with a larger molecular weight, non-aggregated form of hPL. In comparison with the native hormone, the idsplacement curves for the larger form were parallel in radioimmunoassay studies. Sera obtained from pregnant women during various stages of gestation also showed con...Continue Reading

Citations

Sep 21, 1978·The New England Journal of Medicine·J F Habener, J T Potts
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J RussellA B Schneider
Jul 22, 1977·Biochimica Et Biophysica Acta·A B SchneiderL M Sherwood
Aug 1, 1983·British Journal of Obstetrics and Gynaecology·M SideriG Remotti
Apr 1, 1981·Journal of Endocrinological Investigation·A D RogolS W Rosen

Related Concepts

Hydrogen-Ion Concentration
Placenta
Human Placental Lactogen
Prolactin
Recombinant Growth Hormone

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