PMID: 2498276May 1, 1989Paper

Identification of binding protein of virginiae butanolide C, an autoregulator in virginiamycin production, from Streptomyces virginiae

The Journal of Antibiotics
H S KimY Yamada

Abstract

In Streptomyces virginiae, production of virginiamycin is triggered by signal molecules named virginiae butanolide A, B or C (VB-A, B or C: Yamada, Y. et al. J. Antibiotics 40: 496-504, 1987). We have found a specific VB-C binding protein from S. virginiae, and characterized it by using a tritium-labeled VB-C analogue as a ligand. By equilibrium dialysis in the absence and presence of radio-inert VB-C, a crude extract from 1 g of wet mycelia specifically bound 3.5 pmol of [3H]VB. The binding disappeared after pronase digestion and showed ligand specificity toward cis VB-C (cis VB-C greater than trans VB-C much greater than A-factor type), indicating that binding was due to a cis VB-C specific binding protein. Scatchard analysis of the binding demonstrated a single class of high affinity binding sites (Kd 1.1 nM) and low number of the binding sites (30-40 sites/genome DNA). By gel filtration on Sephadex G-75 and molecular sieve HPLC, the binding protein was shown to have an Mr of about 20,000. These results indicate that the substance is a novel VB-C binding protein and suggest that it is a VB-receptor mediating the pleiotropic signal transmitted by VBs in S. virginiae.

Citations

Jan 1, 1991·Critical Reviews in Biotechnology·C M Franco, L E Coutinho
Jan 17, 2012·Applied Microbiology and Biotechnology·Yung-Hun YangByung-Gee Kim
Oct 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R F Rosenzweig, J Adams
Sep 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shigeru KitaniTakuya Nihira
Jan 1, 1990·Critical Reviews in Biotechnology·S Horinouchi, T Beppu
Jan 1, 1993·Antonie van Leeuwenhoek·S Horinouchi, T Beppu
May 24, 2003·Applied Microbiology and Biotechnology·M J ButlerM J Bibb
Oct 31, 1998·Antimicrobial Agents and Chemotherapy·N SuzukiY Yamada
Dec 1, 2020·Frontiers in Bioengineering and Biotechnology·Katsuaki NittaEiichiro Fukusaki
Feb 1, 1995·Journal of Bacteriology·M RuengjitchatchawalyaY Yamada
Nov 1, 1995·Journal of Bacteriology·H OnakaS Horinouchi

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