Identification of biogenic organotellurides in Escherichia coli K-12 headspace gases using solid-phase microextraction and gas chromatography

Analytical Biochemistry
J W SwearingenThomas G Chasteen

Abstract

Escherichia coli JM109 cells, expressing the genes encoded in a 3.8-kb chromosomal DNA fragment from Geobacillus stearothermophilus V, produced volatile organotellurium compounds which were released into the headspace gas above liquid cultures when amended with tellurite anions in micromolar amounts. Headspace sampling was achieved using gas-syringe extraction or solid-phase microextraction using carboxen-polydimethysiloxane fibers. In addition to dimethyl telluride and dimethyl ditelluride, two new organometalloidal compounds were detected using gas chromatograph with mass spectrometric or fluorine-induced chemiluminescence detection. These compounds are methanetellurol and dimethyl tellurenyl sulfide. The significance of these findings with regard to the current knowledge about bacterial tellurite resistance is discussed.

References

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Citations

Jul 27, 2007·Natural Product Reports·Stefan Schulz, Jeroen S Dickschat
Nov 10, 2007·Phytochemistry·Tomás Rezanka, Karel Sigler
Apr 17, 2009·FEMS Microbiology Reviews·Thomas Girard ChasteenClaudio Christian Vásquez
Jan 1, 2010·Chembiochem : a European Journal of Chemical Biology·Jeroen S DickschatNelson L Brock
Dec 3, 2009·Environmental Technology·Radhika BurraThomas G Chasteen

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