Identification of biomarkers for the detection of early stage lung adenocarcinoma by microarray profiling of long noncoding RNAs

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
Ping WangHongju Mao

Abstract

Lung adenocarcinoma has one of the poorest outcomes of any cancer worldwide, in part due to the lack of a reliable means of early detection. Long noncoding RNAs (lncRNAs) have been shown to be deregulated in some types of cancer; however, the contributions of lncRNAs to lung adenocarcinoma remain unknown. We described the expression profile of lncRNAs in human lung adenocarcinoma at an early stage and the corresponding adjacent nontumorous tissues (NT) by microarray. From the microarray analysis, a total of 1170 lncRNAs were significantly differentially expressed in three early stage lung adenocarcinoma tissues compared with NT (fold-change≥2.0, p≤0.05). Candidate biomarkers were selected from the significantly differentially expressed lncRNAs based on our established filtering pipeline; subsequently, marker optimization and validation by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) on a total of 102 pairs of early stage lung adenocarcinoma and NT samples. A panel of 5-lncRNA was identified that could distinguish early stage lung adenocarcinoma from NT samples with high sensitivity and specificity. The area under the receiver operating characteristic curve (AUC) for tumor identification in th...Continue Reading

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Citations

Feb 18, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiaofei ChaiYongping Song
Nov 18, 2015·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Z ShenW Tang
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Apr 25, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jing ZhangXiaodong Chen
Feb 9, 2018·Future Oncology·Cláudia GouvinhasDaniel Humberto Pozza
Oct 20, 2018·Postgraduate Medical Journal·Shui-Ping DaiWei-Min Li
Nov 11, 2019·Advances in Biological Regulation·Javier Arranz-Nicolás, Isabel Mérida

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