PMID: 9185072Apr 1, 1997Paper

Identification of bioneutralization epitopes of human follicle stimulating hormone in the regions 31-52 and 66-75 of its beta-subunit

Journal of Reproductive Immunology
D LalK S Iyer

Abstract

The crucial role played by follicle stimulating hormone (FSH) in regulating both male and female reproduction and the possibilities of developing contraceptive methods for males by blocking the function of the hormone, makes it important to delineate the hormone-specific bioneutralization epitopes of human follicle stimulating hormone (hFSH) on its beta-subunit. Predictive methods were used to identify the potential surface-oriented regions of hFSH-beta. Peptides corresponding to these regions, i.e. 31-52, 66-75 and 86-95 hFSH-beta, were synthesized, anti-peptide antibodies were elicited in rabbits and the properties of these antisera to bind hFSH and neutralize its biological activity were assessed. Anti-31-52 hFSH-beta antisera bound hFSH specifically, whereas anti-66-75 and anti-86-95 hFSH-beta antisera did not show any detectable binding, proving the region 31-52 hFSH-beta to be a specific antigenic determinant of hFSH. The bioneutralizing abilities of the anti-peptide antibodies were assessed by measuring the hFSH-induced progesterone secretion by rat granulosa cells in vitro. Antibodies to 31-52 and 66-75 hFSH-beta neutralized the bioactivity of hFSH, but anti-86-95 hFSH-beta antibodies did not. Furthermore, the three lin...Continue Reading

References

May 1, 1991·International Journal of Peptide and Protein Research·F AlbericioG Barany
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Citations

Oct 9, 1998·American Journal of Reproductive Immunology : AJRI·V A Ferro, W H Stimson
Mar 26, 2015·Applied Biochemistry and Biotechnology·Susha S KutteyilSmita D Mahale
Dec 13, 2007·Journal of Molecular Recognition : JMR·Peter TimmermanRob H Meloen
Apr 23, 2013·Chemical Biology & Drug Design·Rajshri M NavalakheSmita D Mahale
Mar 4, 2000·Endocrine Reviews·M P RoseA H Balen
Jun 11, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Susha S KutteyilSmita D Mahale

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