Identification of bla(CMY-7) and associated plasmid-mediated resistance genes in multidrug-resistant Escherichia coli isolated from dogs at a veterinary teaching hospital in Australia

The Journal of Antimicrobial Chemotherapy
Hanna E SidjabatDarren J Trott

Abstract

To determine clonality and identify plasmid-mediated resistance genes in 11 multidrug-resistant Escherichia coli (MDREC) isolates associated with opportunistic infections in hospitalized dogs in Australia. Phenotypic (MIC determinations, modified double-disc diffusion and isoelectric focusing) and genotypic methods (PFGE, plasmid analysis, PCR, sequencing, Southern hybridization, bacterial conjugation and transformation) were used to characterize, investigate the genetic relatedness of, and identify selected plasmid-mediated antimicrobial resistance genes, in the canine MDREC. Canine MDRECs were divided into two clonal groups (CG 1 and 2) with distinct restriction endonuclease digestion and plasmid profiles. All isolates possessed bla(CMY-7) on an approximately 93 kb plasmid. In CG 1 isolates, bla(TEM), catA1 and class 1 integron-associated dfrA17-aadA5 genes were located on an approximately 170 kb plasmid. In CG 2 isolates, a second approximately 93 kb plasmid contained bla(TEM) and unidentified class 1 integron genes, although a single CG 2 strain carried dfrA5. Antimicrobial susceptibility profiling of E. coli K12 transformed with CG 2 large plasmids confirmed that the bla(CMY-7)-carrying plasmid did not carry any other anti...Continue Reading

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Citations

Jul 24, 2008·Journal of Veterinary Internal Medicine·J S GibsonD J Trott
Mar 1, 2014·Veterinary Microbiology·Joseph E Rubin, Johann D D Pitout
Apr 9, 2014·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Torahiko OkuboYutaka Tamura
Jul 24, 2014·International Journal of Antimicrobial Agents·Hanna E SidjabatDavid L Paterson
Apr 19, 2011·Veterinary Microbiology·Peter DamborgLuca Guardabassi
Jan 18, 2011·The Veterinary Journal·Mary F ThompsonDarren J Trott
Apr 29, 2015·Antimicrobial Agents and Chemotherapy·Hanna E SidjabatDavid L Paterson
Jul 25, 2014·Journal of Clinical Microbiology·Hanna E SidjabatDavid L Paterson
Oct 16, 2015·Antimicrobial Agents and Chemotherapy·Thouraya ChérifGuillaume Arlet
Apr 17, 2019·Journal of Medical Microbiology·Megha KaushikPooja Gulati
Sep 15, 2018·The Journal of Antimicrobial Chemotherapy·Vanessa M SchmidtNicola J Williams
Jul 14, 2018·Microbiology Spectrum·Laurent PoirelStefan Schwarz

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