Identification of both Gi2 and a novel, immunologically distinct, form of Go in rat myometrial membranes

FEBS Letters
G MilliganS Harbon

Abstract

Immunoblotting of rat myometrial membranes with an antiserum (SG1) which recognizes the alpha-subunits of both Gi1 and Gi2 indicated the presence of detectable levels of an apparently single form of some 40 kDa. A second antiserum (LE2) specific for Gi2 also recognized this protein, confirming its identity. Immunoblotting of the myometrial membranes with a series of antipeptide (OC1, IM1, ON1) and polyclonal (RV3) antisera, all of which recognize rat brain Go, produced a more complex pattern. Antisera OC1 and ON1 recognized a single polypeptide which essentially comigrated with rat brain Go. In contrast, antisera RV3 and IM1 did not recognize the myometrial protein. These data provide evidence for the presence of Gi2 and of a novel G-protein, related immunologically to Go, in rat myometrial membranes.

References

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Citations

Dec 15, 1994·European Journal of Pharmacology·R ZumbihlB Rouot
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M StrathmannM I Simon
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T TsukamotoY Kaziro
Feb 13, 2009·Neuro-Signals·Meisheng Jiang, Neil S Bajpayee
Jul 1, 1997·Experimental Parasitology·K R GrantW Harnett
Sep 21, 1992·FEBS Letters·M I Sweeney, A C Dolphin
Mar 10, 2007·Human Reproduction·Kati S MönkkönenAlireza Fazeli

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