Abstract
Immunoblotting of rat myometrial membranes with an antiserum (SG1) which recognizes the alpha-subunits of both Gi1 and Gi2 indicated the presence of detectable levels of an apparently single form of some 40 kDa. A second antiserum (LE2) specific for Gi2 also recognized this protein, confirming its identity. Immunoblotting of the myometrial membranes with a series of antipeptide (OC1, IM1, ON1) and polyclonal (RV3) antisera, all of which recognize rat brain Go, produced a more complex pattern. Antisera OC1 and ON1 recognized a single polypeptide which essentially comigrated with rat brain Go. In contrast, antisera RV3 and IM1 did not recognize the myometrial protein. These data provide evidence for the presence of Gi2 and of a novel G-protein, related immunologically to Go, in rat myometrial membranes.
References
Jan 4, 1987·Nature·J HeschelerG Schultz
Jan 1, 1987·Molecular and Cellular Endocrinology·A M Spiegel
Jul 15, 1987·The Biochemical Journal·G Milligan
Feb 1, 1988·The Biochemical Journal·F R McKenzieG Milligan
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
Jan 20, 1986·FEBS Letters·G MilliganW A Klee
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P GierschikA Spiegel
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H ItohK Suzuki
Jan 26, 1987·FEBS Letters·J R DidsburyR Snyderman
Feb 1, 1987·Biochemical Society Transactions·G MilliganA M Spiegel
May 21, 1987·Nature·D GawlerM D Houslay
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P BrayM Nirenberg
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Oct 1, 1987·The Biochemical Journal·G MilliganE D Saggerson
Apr 15, 1988·Biochemical and Biophysical Research Communications·H McArdleG Milligan
Sep 1, 1988·The Biochemical Journal·G MilliganH McArdle
Sep 6, 1988·Biochemistry·P GoldsmithA Spiegel
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M PinesA Spiegel
Citations
Aug 15, 1995·European Journal of Pharmacology·J B Calixto
Sep 3, 1991·Biochimica Et Biophysica Acta·J L GaribayY Kaziro
Feb 1, 1993·Neuroscience·E HustonA C Dolphin
Jan 1, 1990·Cellular Signalling·K G Macleod, G Milligan
Dec 15, 1994·European Journal of Pharmacology·R ZumbihlB Rouot
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M StrathmannM I Simon
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T TsukamotoY Kaziro
Mar 11, 1994·Nucleic Acids Research·J J MurtaghM Vaughan
Feb 13, 2009·Neuro-Signals·Meisheng Jiang, Neil S Bajpayee
Jul 1, 1997·Experimental Parasitology·K R GrantW Harnett
Mar 30, 1990·Biochemical and Biophysical Research Communications·C LiebmannW Rosenthal
Sep 21, 1992·FEBS Letters·M I Sweeney, A C Dolphin
Mar 10, 2007·Human Reproduction·Kati S MönkkönenAlireza Fazeli
Jul 24, 1998·Biochimica Et Biophysica Acta·L GamblingD L Baines
Jul 9, 1999·Biochimica Et Biophysica Acta·L GamblingD L Baines
Feb 11, 1991·Molecular and Cellular Biology·J J MurtaghM Vaughan