Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry

Proteomics
Da-Qiang LiZhi-Min Shao

Abstract

To better understand the molecular mechanisms underlying breast cancer metastasis and search for potential markers for metastatic progression, we have developed a highly metastatic variant of human MDA-MB-435 breast cancer cell line through in vivo stepwise selection of pulmonary metastatic cells caused by parental MDA-MB-435 cells in the athymic mice. Comparative proteomic analysis using 2-DE and LC-IT-MS revealed that 102 protein spots were reproducibly altered more than three-fold between the selected variant and its parental counterpart. Eleven differentially expressed protein spots were identified with high confidence using SEQUEST with uninterpreted tandem mass raw data. Cathepsin D precursor, peroxiredoxin 6 (PDX6), heat shock protein 27 (HSP27), HSP60, tropomyosin 1 (TPM1), TPM2, TPM3, TPM4, 14-3-3 protein epsilon, and tumor protein D54 were up-regulated in the highly metastatic variant, whereas alpha B-crystalline (CRAB) was only detected in its parental counterpart. Differential expression was confirmed for four proteins including PDX6, CRAB, TPM4, and HSP60 by real-time quantitative PCR and Western blotting analysis in our model. Immunohistochemical analysis in 80 breast cancer donors demonstrated a significant assoc...Continue Reading

References

Oct 20, 1995·Science·V E VelculescuK W Kinzler
Feb 12, 1993·Science·N LisitsynM Wigler
Apr 1, 1996·British Journal of Cancer·B FranzénG Auer
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Jan 1, 1996·Breast Cancer Research and Treatment·J E Price
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·M E RayJ M Trent
Apr 16, 1998·Protein Science : a Publication of the Protein Society·A DucretR Aebersold
Oct 7, 1998·International Journal of Cancer. Journal International Du Cancer·L M Vargas-RoigD R Ciocca
Feb 18, 1999·Molecular and Cellular Biology·S P GygiR Aebersold
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·H FuS C Masters
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P GygiR Aebersold
Feb 2, 2002·Nature·Laura J van 't VeerStephen H Friend
Sep 6, 2002·Experimental Cell Research·Kalyankar MahadevG L Prasad
Mar 14, 2003·Nature·Sam Hanash
Jul 29, 2003·Journal of Mammary Gland Biology and Neoplasia·Hubert HondermarckJérôme Lemoine
Oct 2, 2003·International Journal of Cancer. Journal International Du Cancer·Hyerim LeeJeffrey W Smith
Feb 6, 2004·Breast Cancer Research and Treatment·Marc Lacroix, Guy Leclercq
Jul 10, 2004·Journal of Cancer Research and Clinical Oncology·Jie-Feng CuiPeng-Yuan Yang
Jan 22, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Kyoko Iwao-KoizumiKikuya Kato
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·J K EngJ R Yates

❮ Previous
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Citations

Oct 12, 2007·Breast Cancer Research and Treatment·Xin-Zhong ChangZhi-Ming Shao
Apr 12, 2011·Breast Cancer Research and Treatment·Ji-Yu LiZhi-Ming Shao
Oct 10, 2009·Molecular & Cellular Proteomics : MCP·Bai-Yu LeeMarcus A Horwitz
Nov 6, 2007·Breast Cancer Research : BCR·Xin-Zhong ChangZhi-Ming Shao
Mar 27, 2012·Journal of Clinical Biochemistry and Nutrition·Tetsuro IshiiToru Yanagawa
Dec 12, 2012·Sarcoma·Yoshiyuki SueharaTadashi Kondo
Jan 22, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Maria KabbageKarim Chahed
Jul 3, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Maria KabbageKarim Chahed
Sep 14, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Maria KabbageKarim Chahed
Oct 15, 2008·Journal of Cancer Research and Clinical Oncology·Hui-zhi FanPeng-yuan Yang
Apr 6, 2011·Journal of Cancer Research and Clinical Oncology·E R C G N GalvãoS J H Monte
Jan 8, 2016·Cancer Immunology, Immunotherapy : CII·Michael J CampaEdward F Patz
Feb 5, 2013·Tissue & Cell·Mohamad Zamani-AhmadmahmudiBabak Yazdani
Jun 9, 2009·Cancer Letters·Bo ZhangYongping Su
Apr 22, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bechr HamritaLotfi Chouchane
Jan 31, 2007·Journal of Mass Spectrometry : JMS
Aug 5, 2008·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Lei ZhangZhi-yuan Zhang
Jan 20, 2009·International Journal of Cancer. Journal International Du Cancer·Yazhou CuiJinxiang Han
Jul 23, 2013·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Bo ZhangYun Bai
Oct 21, 2010·Proteomics·Hexin ChenAkhilesh Pandey

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