Identification of budesonide metabolites in human urine after oral administration

Analytical and Bioanalytical Chemistry
Xavier MataboschRosa Ventura

Abstract

Budesonide (BUD) is a glucocorticoid widely used for the treatment of asthma, rhinitis, and inflammatory bowel disease. Its use in sport competitions is prohibited when administered by oral, intravenous, intramuscular, or rectal routes. However, topical preparations are not prohibited. Strategies to discriminate between legal and forbidden administrations have to be developed by doping control laboratories. For this reason, metabolism of BUD has been re-evaluated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with different scan methods. Urine samples obtained after oral administration of 3 mg of BUD to two healthy volunteers have been analyzed for metabolite detection in free and glucuronide metabolic fractions. Structures of the metabolites have been studied by LC-MS/MS using collision induced dissociation and gas chromatography-mass spectrometry (GC/MS) in full scan mode with electron ionization. Combination of all structural information allowed the proposition of the most comprehensive picture for BUD metabolism in humans to this date. Overall, 16 metabolites including ten previously unreported compounds have been detected. The main metabolite is 16α-hydroxy-prednisolone resulting from the cleavage of the a...Continue Reading

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Citations

Oct 8, 2014·Analytical and Bioanalytical Chemistry·Adrian A AmmannMarc J F Suter
Nov 1, 2013·Drug Metabolism Reviews·Petra Malátková, Vladimír Wsól
Jun 6, 2012·The Journal of Steroid Biochemistry and Molecular Biology·C GómezR Ventura
Dec 29, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Josep Marcos, Oscar J Pozo
Mar 25, 2015·Journal of Mass Spectrometry : JMS·Xavier MataboschRosa Ventura
Dec 30, 2014·Drug Testing and Analysis·Xavier MataboschRosa Ventura
Sep 28, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Xavier MataboschRosa Ventura
Feb 28, 2019·Expert Opinion on Drug Discovery·Javier Saurina, Sonia Sentellas
Nov 9, 2019·Drug Testing and Analysis·Monica MazzarinoFrancesco Botrè
Sep 14, 2019·The European Respiratory Journal·Paul BrinkmanUNKNOWN U-BIOPRED Study Group
Jan 16, 2013·Therapeutic Drug Monitoring·Xavier MataboschRosa Ventura
Jul 6, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Amanda Lessa Dutra de AraujoCarina de Souza Anselmo
Aug 15, 2021·Journal of Pharmaceutical and Biomedical Analysis·Mario ThevisAndreas Thomas

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