Identification of C4 responsive genes in the facultative C4 plant Hydrilla verticillata.

Photosynthesis Research
Srinath K RaoG Bowes

Abstract

The aquatic monocot Hydrilla verticillata (L.f.) Royle is a well-documented facultative C4 NADP-malic enzyme species in which the C4 and Calvin cycles operate in the same cell with the specific carboxylases confined to the cytosol and chloroplast, respectively. Several key components had already been characterized at the molecular level, thus the purpose of this study was to begin to identify other, less obvious, elements that may be necessary for a functional single-cell C4 system. Using differential display, mRNA populations from C3 and C4 H. verticillata leaves were screened and expression profiles compared. From this study, 65 clones were isolated and subjected to a customized macroarray analysis; 25 clones were found to be upregulated in C4 leaves. Northern and semi-quantitative RT-PCR analyses were used for confirmation. From these screenings, 13 C4 upregulated genes were identified. Among these one encoded a previously recognized C4 phosphoenolpyruvate carboxylase, and two encoded distinct pyruvate orthophosphate dikinase isoforms, new findings for H. verticillata. Genes that encode a transporter, an aminotransferase and two chaperonins were also upregulated. Twelve false positives, mostly housekeeping genes, were determ...Continue Reading

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Citations

Apr 5, 2011·Journal of Experimental Botany·Mitsue MiyaoHiroshi Fukayama
Jan 10, 2018·Journal of Occupational Health·Eman A Salem, Sabah M Ebrahem
Sep 14, 2017·International Journal of Genomics·Shiqi HuHongwei Hou

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