Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera

FEBS Letters
D DuplatE Lopez

Abstract

Nacre or mother-of-pearl in the shell of Pinctada margaritifera is composed of 95-99% calcium carbonate and 1-5% organic matrix. In this study, we developed an original technique to characterize the genes differentially expressed in nacre-forming cells (NFC) by combining suppression subtractive hybridization (SSH), to establish a cDNA subtractive library, with rapid amplification of cDNA ends (RACE)-PCR. Seventy-two specific cDNA sequences have been obtained so far. These include a protein containing two EF-hand Ca2+-binding domains which was completely sequenced after amplification by RACE-PCR. Its specific expression as well as the specificity of the SSH method was confirmed by semi-quantitative RT-PCR on NFC and mantle cells.

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Citations

Nov 24, 2006·BMC Biology·Daniel J JacksonBernard M Degnan
Apr 25, 2007·Biochimica Et Biophysica Acta·Jing HuangRongqing Zhang
Jul 29, 2011·Chembiochem : a European Journal of Chemical Biology·C MontagnaniN Cochennec-Laureau
Nov 11, 2009·Acta Biochimica Et Biophysica Sinica·Qiaoli FengRongqing Zhang
Mar 16, 2011·Chembiochem : a European Journal of Chemical Biology·Sophie BerlandLaurent Bédouet

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